CaSBP11 Participates in the Defense Response of Pepper to Phytophthora capsici through Regulating the Expression of Defense-Related Genes

被引:15
|
作者
Zhang, Huai-Xia [1 ,2 ]
Feng, Xiao-Hui [1 ]
Jin, Jing-Hao [1 ]
Khan, Abid [3 ]
Guo, Wei-Li [2 ]
Du, Xiao-Hua [2 ]
Gong, Zhen-Hui [1 ]
机构
[1] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
[2] Henan Inst Sci & Technol, Sch Hort Landscape Architecture, Xinxiang 453003, Henan, Peoples R China
[3] Univ Haripur, Dept Hort, Haripur 22620, Pakistan
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
pepper; CaSBP11; Phytophthora capsici; defense-related genes; Nicotiana benthamiana; SBP-BOX GENE; GENOME-WIDE IDENTIFICATION; SALICYLIC-ACID; TRANSCRIPTION FACTOR; DIFFERENTIAL EXPRESSION; ENVIRONMENTAL-STRESSES; PATHOGEN INFECTION; ABIOTIC ELICITORS; VEGETABLE CROPS; FAMILY;
D O I
10.3390/ijms21239065
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Squamosa promoter binding protein (SBP)-box genes are plant-specific transcription factors involved in plant growth and development, morphogenesis and biotic and abiotic stress responses. However, these genes have been understudied in pepper, especially with respect to defense responses to Phytophthora capsici infection. CaSBP11 is a SBP-box family gene in pepper that was identified in our previous research. Silencing CaSBP11 enhanced the defense response of pepper plants to Phytophthora capsici. Without treatment, the expression of defense-related genes (CaBPR1, CaPO1, CaSAR8.2 and CaDEF1) increased in CaSBP11-silenced plants. However, the expression levels of these genes were inhibited under transient CaSBP11 expression. CaSBP11 overexpression in transgenic Nicotiana benthamiana decreased defense responses, while in Arabidopsis, it induced or inhibited the expression of genes in the salicylic acid and jasmonic acid signaling pathways. CaSBP11 overexpression in sid2-2 mutants induced AtNPR1, AtNPR3, AtNPR4, AtPAD4, AtEDS1, AtEDS5, AtMPK4 and AtNDR1 expression, while AtSARD1 and AtTGA6 expression was inhibited. CaSBP11 overexpression in coi1-21 and coi1-22 mutants, respectively, inhibited AtPDF1.2 expression and induced AtPR1 expression. These results indicate CaSBP11 has a negative regulatory effect on defense responses to Phytophthora capsici. Moreover, it may participate in the defense response of pepper to Phytophthora capsici by regulating defense-related genes and the salicylic and jasmonic acid-mediated disease resistance signaling pathways.
引用
收藏
页码:1 / 22
页数:21
相关论文
共 50 条
  • [41] Coordinated expression of defense-related genes by TMV infection or salicylic acid treatment in tobacco
    Kang, MK
    Park, KS
    Choi, D
    MOLECULES AND CELLS, 1998, 8 (04) : 388 - 392
  • [42] Expression of defense-related genes in the incompatible reaction between wheatand Tilletia tritici.
    不详
    CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE, 2004, 26 (03): : 410 - 411
  • [43] Temporal expression of defense-related genes in Ganoderma-infected oil palm roots
    Mohan, Durgadevi
    Namasivayam, Parameswari
    Saidi, Noor Baity
    Ho, Chai-Ling
    TREES-STRUCTURE AND FUNCTION, 2022, 36 (03): : 1029 - 1038
  • [44] Expression analysis of defense-related genes in cucumber (Cucumis sativusL.) againstPhytophthora melonis
    Hashemi, Lida
    Golparvar, Ahmad Reza
    Nasr-Esfahani, Mehdi
    Golabadi, Maryam
    MOLECULAR BIOLOGY REPORTS, 2020, 47 (07) : 4933 - 4944
  • [45] Transcriptome characterization and expression profile of defense-related genes in pear induced by Meyerozyma guilliermondii
    Yan, Yuan
    Zheng, Xiangfeng
    Apaliya, Maurice Tibiru
    Yang, Hongjuan
    Zhang, Hongyin
    POSTHARVEST BIOLOGY AND TECHNOLOGY, 2018, 141 : 63 - 70
  • [46] Isolation and expression analysis of defense-related genes in sorghum-Colletotrichum sublineolum interaction
    Li, Lei
    Zhu, Fuyuan
    Liu, Hongjia
    Chu, Apple
    Lo, Clive
    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2013, 84 : 123 - 130
  • [47] A histone deacetylase inhibitor enhances rice immunity by derepressing the expression of defense-related genes
    Xu, Yan
    Miao, Yuanxin
    Cai, Botao
    Yi, Qingping
    Tian, Xuejun
    Wang, Qihai
    Ma, Dan
    Luo, Qiong
    Tan, Feng
    Hu, Yongfeng
    FRONTIERS IN PLANT SCIENCE, 2022, 13
  • [48] Silencing of defense-related genes reveals different mechanisms leading to race-specific resistance to Phytophthora in soybean
    Graham, M. Y.
    Sinden, M. R.
    Huge, R.
    Subramanian, S.
    Yu, O.
    Martin, S. St.
    Graham, T. L.
    PHYTOPATHOLOGY, 2008, 98 (06) : S61 - S61
  • [49] Expression of defense-related genes in mung bean varieties in response to Trichoderma virens alone and in the presence of Rhizoctonia solani infection
    Sunil C. Dubey
    Aradhika Tripathi
    Rakesh Tak
    3 Biotech, 2018, 8
  • [50] Effects of baobab (Adansonia digitata L.) in combination with Sporidiobolus pararoseus Y16 on the activities of the defense-related enzymes and the expression levels of defense-related genes of apples
    Abdelhai, Mandour H.
    Zhang, Qiru
    Zhao, Lina
    Mahunu, Gustav Komla
    Musa, Abubakr
    Yang, Qiya
    Serwah, Nana Adwoa
    Zhang, Hongyin
    BIOLOGICAL CONTROL, 2019, 139