Structure of the 70S ribosome from human pathogen Staphylococcus aureus

被引：44

作者：

Khusainov, Iskander ^{[1
,2
]}

Vicens, Quentin ^{[3
]}

Bochler, Anthony ^{[3
]}

Grosse, Francois ^{[3
]}

Myasnikov, Alexander ^{[1
]}

Menetret, Jean-Francois ^{[1
]}

Chicher, Johana ^{[3
]}

Marzi, Stefano ^{[3
]}

Romby, Pascale ^{[3
]}

Yusupova, Gulnara ^{[1
]}

Yusupov, Marat ^{[1
]}

Hashem, Yaser ^{[3
]}

机构：

[1] Univ Strasbourg, Dept Biol & Genom Struct, Inst Genet & Biol Mol & Cellulaire, CNRS UMR7104,INSERM U964, F-67400 Illkirch Graffenstaden, France

[2] Kazan Fed Univ, Inst Fundamental Med & Biol, Kazan 420008, Russia

[3] Univ Strasbourg, Architecture & Reactivite ARN, Inst Biol Mol & Cellulaire, CNRS, F-67084 Strasbourg, France

来源：

NUCLEIC ACIDS RESEARCH | 2016年 / 44卷 / 21期

基金：

俄罗斯科学基金会;

关键词：

HIGH-RESOLUTION STRUCTURE; ESCHERICHIA-COLI; MESSENGER-RNA; PROTEIN S1; BACILLUS-SUBTILIS; CRYO-EM; SPECIALIZED RIBOSOMES; STRUCTURE VALIDATION; VIRULENCE FACTORS; CRYSTAL-STRUCTURE;

D O I：

10.1093/nar/gkw933

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely related organisms, their existence could account for vital yet species-specific mechanisms of translation regulation that would involve stalling, cell survival and antibiotic resistance. Here, we present the first full 70S ribosome structure from Staphylococcus aureus, a Gram-positive pathogenic bacterium, solved by cryo-electron microscopy. Comparative analysis with other known bacterial ribosomes pinpoints several unique features specific to S. aureus around a conserved core, at both the protein and the RNA levels. Our work provides the structural basis for the many studies aiming at understanding translation regulation in S. aureus and for designing drugs against this often multi-resistant pathogen.

引用

页码：10491 / 10504

页数：14

共 106 条

[1] A pattern matching approach to the automatic selection of particles from low-contrast electron micrographs [J].