Molecular and physiological analysis of drought stress responses in Zea mays treated with plant growth promoting rhizobacteria

被引：16

作者：

Ahmad, I. ^{[1
,5
]}

Zaib, S. ^{[1
]}

Alves, P. C. M. S. ^{[2
]}

Luthe, D. S. ^{[3
]}

Bano, A. ^{[4
]}

Shakeel, S. N. ^{[1
]}

机构：

[1] Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad 45320, Pakistan

[2] Univ Fed Lavras, Dept Plant Biotechnol, BR-3720000 Lavras, Brazil

[3] Penn State Univ, Coll Agr Sci, University Pk, PA 16802 USA

[4] Quaid I Azam Univ, Fac Biol Sci, Dept Plant Sci, Islamabad 45320, Pakistan

[5] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA

来源：

BIOLOGIA PLANTARUM | 2019年 / 63卷

关键词：

chlorophyll; gene expression; maize; protein; Pseudomonas fluorescens; Pseudomonas putida; WATER-DEFICIT STRESS; OXIDATIVE STRESS; ABIOTIC STRESS; JASMONIC ACID; ROOT DEVELOPMENT; GENE-EXPRESSION; TOLERANCE; MECHANISMS; LEAF; L;

D O I：

10.32615/bp.2019.092

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Our research intended to appraise the performance of two different Pseudomonas strains on Zea mays L. (cv. B73) under drought stress and non-stress conditions. Plants were inoculated with P. putida KT2440 (Pp) and P. fluorescens (Pf1) followed by sampling at 0, 3rd, and 6th day after imposition of drought stress (DAS). Both strains demonstrated significant improvement in root length, protein content, chlorophyll content, and root and shoot fresh masses as compared to un-inoculated drought stressed plants. Real-time quantitative PCR analysis revealed that drought stress responsive genes, i.e., the cold-related dehydrin 410 gene, WRKY18, and major facilitator superfamily were significantly down-regulated by Pf1 and Pp inoculation under drought stress condition on 6 DAS. Similarly, the down-regulated transcript abundance of lipoxygenase genes in inoculated plants on 6 DAS showed the role of Pf1 and Pp in scavenging reactive oxygen species under drought stress conditions. Among the selected jasmonic acid pathway responsive genes, maize protease inhibitor and 12-oxo-phytodienoatereductase 7 (OPR7) also revealed a potential role of these rhizobacteria under drought stress conditions. Seed inoculation of both strains significantly down-regulated the expression of OPR7 gene under stress conditions. Our results advocate the complex growth promotion effects of both selected rhizobacterial strains and amelioration of the drought by modulating the expression of drought stress responsive genes.

引用

页码：536 / 547

页数：12

共 64 条

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