Anti-allergic inflammatory components from Sanguisorba officinalis L.

Sanguisorba officinalis L. was well known as a traditional herbal medicine to treat inflammation and allergic skin diseases. The aim of this research was to identify compounds with anti-allergic inflammatory property. Twenty-five compounds (1-25) were isolated from S.officinalis including two new compounds (1 and 8), and their chemical structures were identified by NMR and ESIMS analysis. Consequently, the anti-allergic inflammatory activities of these isolates were investigated by inhibiting p-hexosaminidase and IL-4 production in PMA/A23187-stimulated RBL-2H3 cells. Compounds 6, 8,13,17-18 and 25 significantly inhibited p-hexosaminidase release and IL-4 production. Additionally, compounds 8, 17 and 25 effectively suppressed the activation of NF-kappa B and NF-kappa B p65 translocation into the nucleus. Anti-inflammatory effects of isolated compounds were evaluated in LPS-stimulated RAW264.7 macrophages, and they showed dramatic inhibition on LPS-induced overproduction of nitric oxide (NO) and TNF-a. Consistently, the protein levels of iNOS and COX-2 were remarkably decreased by the single compounds 8,13 and 25. These results showed that compounds 8,13 and 25 from S.officinalis may have a therapeutic potential for allergic inflammatory diseases. (C) 2018 Elsevier Ltd. All rights reserved.