Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines

被引：9
|
作者：
Kim, Younghoon [1 ]
Kim, Mae Jin [1 ]
Han, Kyoung Sik [2 ]
Imm, Jee Young [3 ]
Oh, Sejong [4 ]
Kim, Sae Hun [1 ]
机构：
[1] Korea Univ, Coll Life Sci & Biotechnol, Div Food Biosci & Technol, Seoul 136701, South Korea
[2] Massey Univ, Riddet Ctr, Palmerston North, New Zealand
[3] Kookmin Univ, Dept Food & Nutr, Seoul 136702, South Korea
[4] Chonnam Natl Univ, Dept Anim Sci, Kwangju 500757, South Korea
来源：
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY | 2009年 / 62卷 / 02期
关键词：
Caprine lactoferrin; Chromatography; Purification; Human cancer cell lines; Anticancer activity; NATIVE GOAT LACTOFERRIN; BOVINE LACTOFERRIN; ANTIBACTERIAL PEPTIDES; IN-VITRO; SEQUENCE; BINDING;
D O I：
10.1111/j.1471-0307.2009.00466.x
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In the present study, lactoferrin was purified from caprine colostrum and its anticancer effects were investigated on various cancer cell lines, including lung, colon, cervix, stomach and breast cancer cells. The caprine lactoferrin (CLF) was purified by ultrafiltration and sequential chromatography using a CM-Sephadex C-50-120 ion-exchange column and affinity chromatography on Hi-Trap(TM) Heparin HP. The purified CLF exhibited antiproliferative effects on the five tested cancer cell lines in a dose-dependent manner, with 20-30% survival as compared to the control. Interestingly, the most intense inhibition of cell growth was observed on the ZR-75-1 breast cancer cells (IC50 = 27.5 mu g/mL). Our findings suggest, for the first time, that purified CLF from caprine colostrum may constitute a novel anticancer agent for the food industry.
引用
收藏
页码：277 / 281
页数：5
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