Development of a real-time quantitative polymerase chain reaction assay for the detection of congenital human cytomegalovirus infection in urine samples

被引：6

作者：

Ligozzi, Marco ^{[1
]}

Poggi, Mara ^{[2
]}

Saletti, Marilena ^{[2
]}

Gibellini, Davide ^{[1
]}

机构：

[1] Univ Verona, Dept Pathol & Diagnost, Microbiol & Virol Unit, Str Grazie 8, I-37134 Verona, Italy

[2] Azienda Osped Univ Integrata Verona, Dept Pathol, Microbiol & Virol Unit, Piazzale Stefani 10, I-35124 Verona, Italy

来源：

MOLECULAR AND CELLULAR PROBES | 2016年 / 30卷 / 01期

关键词：

HCMV; PCR; Shell vial viral culture; Congenital infection; ORGAN TRANSPLANT RECIPIENTS; ANTIGENEMIA ASSAY; PCR; DNA; DIAGNOSIS; AMPLIFICATION; MANAGEMENT; CULTURE; QUANTIFICATION; NEWBORNS;

D O I：

10.1016/j.mcp.2015.11.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A TaqMan real-time quantitative PCR (qPCR), based on amplification of HCMV UL54 gene specific sequence, was developed and compared with shell vial viral culture assay, the gold standard technique for the diagnosis of congenital HCMV infection, using urine samples collected from 110 newborns. The results indicate that this qPCR is slightly more sensitive than shell vial assay suggesting that qPCR may be considered a useful alternative for diagnosing congenital HCMV infection. (C) 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.orgilicensesiby/4.0/).

引用

页码：50 / 52

页数：3

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