Characterisation of stress protein LysU. Enzymic synthesis of diadenosine 5',5'''-P-1,P-4-tetraphosphate (Ap(4)A) analogues by LysU

被引：15

作者：

Theoclitou, ME ^{[1
]}

Wittung, EPL ^{[1
]}

Hindley, AD ^{[1
]}

ElThaher, TSH ^{[1
]}

Miller, AD ^{[1
]}

机构：

[1] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT CHEM,LONDON SW7 2AY,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | 1996年 / 16期

关键词：

D O I：

10.1039/p19960002009

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The stress protein LysU (lysyl tRNA synthetase) has been purified from a recombinant strain of Escherichia coli expressing the plasmid pXLys5, and kinetically characterised. Preparative syntheses of analogues of the biologically important molecule diadenosine 5',5'''-P-1,P-4-tetraphosphate (Ap(4)A) are then achieved in good yield by enzyme catalysis, using purified LysU.

引用

页码：2009 / 2019

页数：11

共 50 条

[1]

ARMITAGE IM, 1983, NMR NEWLY ACCESSIBLE, P337

[2] ALTERATION OF ADENYL DINUCLEOTIDE METABOLISM BY ENVIRONMENTAL-STRESS [J].

BAKER, JC ;

JACOBSON, MK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (08) :2350-2352

[3]

Bertini I, 1984, Adv Inorg Biochem, V6, P71

[4] APPPPA AND RELATED ADENYLYLATED NUCLEOTIDES ARE SYNTHESIZED AS A CONSEQUENCE OF OXIDATION STRESS [J].

BOCHNER, BR ;

LEE, PC ;

WILSON, SW ;

CUTLER, CW ;

AMES, BN .

CELL, 1984, 37 (01) :225-232

[5] ESCHERICHIA-COLI DNAK PROTEIN POSSESSES A 5'-NUCLEOTIDASE ACTIVITY THAT IS INHIBITED BY APPPPA [J].

BOCHNER, BR ;

ZYLICZ, M ;

GEORGOPOULOS, C .

JOURNAL OF BACTERIOLOGY, 1986, 168 (02) :931-935

[6] COMPARISON OF THE ENZYMATIC-PROPERTIES OF THE 2 ESCHERICHIA-COLI LYSYL-TRANSFER-RNA SYNTHETASE SPECIES [J].

BREVET, A ;

CHEN, J ;

LEVEQUE, F ;

BLANQUET, S ;

PLATEAU, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (24) :14439-14444

[7] INVIVO SYNTHESIS OF ADENYLYLATED BIS(5'-NUCLEOSIDYL) TETRAPHOSPHATES (AP4N) BY ESCHERICHIA-COLI AMINOACYL-TRANSFER RNA-SYNTHETASES [J].

BREVET, A ;

CHEN, J ;

LEVEQUE, F ;

PLATEAU, P ;

BLANQUET, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8275-8279

[8]

BREVET A, 1985, J BIOL CHEM, V260, P5566

[9] THE ACTIVE-SITE OF YEAST ASPARTYL-TRANSFER-RNA SYNTHETASE - STRUCTURAL AND FUNCTIONAL-ASPECTS OF THE AMINOACYLATION REACTION [J].

CAVARELLI, J ;

ERIANI, G ;

REES, B ;

RUFF, M ;

BOEGLIN, M ;

MITSCHLER, A ;

MARTIN, F ;

GANGLOFF, J ;

THIERRY, JC ;

MORAS, D .

EMBO JOURNAL, 1994, 13 (02) :327-337

[10] LYSYL-TRANSFER RNA-SYNTHETASE FROM ESCHERICHIA-COLI-K12 - CHROMATOGRAPHIC HETEROGENEITY AND THE LYSU-GENE PRODUCT [J].

CHARLIER, J ;

SANCHEZ, R .

BIOCHEMICAL JOURNAL, 1987, 248 (01) :43-51

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