Role of endogenous lipids in the chromophore regeneration of bacteriorhodopsin

被引：6
作者：
Catucci, L
Lattanzio, VMT
Lobasso, S
Agostiano, A
Corcelli, A
机构：
[1] Univ Bari, Dipartimento Fisiol Gen & Ambientale, I-70126 Bari, Italy
[2] Univ Bari, Dipartimento Chim, I-70126 Bari, Italy
来源：
BIOELECTROCHEMISTRY | 2004年 / 63卷 / 1-2期
关键词：
bacteriorhodopsin; chromophore regeneration; glycocardiolipin;
D O I：
10.1016/j.bioelechem.2003.09.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The regeneration method of Khorana [J. Biol. Chem. 262 (1987) 9271] has been modified in order to study the effect of endogenous archaeabacterial lipids and, in particular, of glycocardiolipin (GlyC) in the refolding and chromophore regeneration of bacteriorhodopsin (BR). BR refolding and chromophore regeneration could be obtained in the presence of endogenous lipid mixtures containing or not containing glycocardiolipin; however, the kinetics of bacteriorhodopsin regeneration in the presence of glycocardiolipin was faster than in its absence. These results show for the first time that the interaction of glycocardiolipin with bacteriorhodopsin favours its refolding from the denaturated state and the chromophore regeneration. (C) 2004 Elsevier B.V. All rights reserved.
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收藏
页码：111 / 115
页数：5
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