Development and Validation of a HPTLC Method for the Estimation of Sumatriptan in Tablet Dosage Forms

被引：12

作者：

Shah, C. R. ^{[1
]}

Suhagia, B. N. ^{[2
]}

Shah, N. J. ^{[1
]}

Shah, R. R. ^{[3
]}

机构：

[1] Shri BM Shah Coll Pharmaceut Educ & Res, Modasa 383315, India

[2] LM Coll Pharm, Ahmadabad 380009, Gujarat, India

[3] Swaminarayan Sanskar Pharm Coll, Zundal 380009, India

来源：

INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2008年 / 70卷 / 06期

关键词：

HPTLC; sumatriptan; validation;

D O I：

10.4103/0250-474X.49138

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of sumatriptan in tablet dosage forms. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of methanol: water: glacial acetic acid (4.0:8.0:0.1, v/v/v). The detection of spots was carried out at 230 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 800 ng/spot. The limit of detection and the limit of quantification for the sumatriptan were found to be 63.87 and 193.54 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.

引用

页码：831 / 834

页数：4