Cellular uptake, cytotoxicity, apoptosis and DNA-binding investigations of Ru(II) complexes

被引：32

作者：

Devi, C. Shobha ^{[1
]}

Nagababu, Penumaka ^{[1
]}

Natarajan, Sumathi ^{[2
]}

Deepika, N. ^{[1
]}

Reddy, P. Venkat ^{[1
]}

Veerababu, N. ^{[2
]}

Singh, Surya S. ^{[2
]}

Satyanarayana, S. ^{[1
]}

机构：

[1] Osmania Univ, Dept Chem, Hyderabad 500007, Andhra Pradesh, India

[2] Osmania Univ, Dept Biochem, Hyderabad 500007, Andhra Pradesh, India

来源：

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY | 2014年 / 72卷

关键词：

DNA binding; Fluorescence; Cellular uptake; Cytotoxicity; Apoptosis; MIXED-LIGAND COMPLEXES; LUMINESCENCE; EXPRESSION; DRUGS; ASSAY; ACID;

D O I：

10.1016/j.ejmech.2013.11.005

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Three new compounds, [Ru(Hdpa)(2)PyIP](ClO4)(2)center dot 2H(2)O (1) [Ru(Hdpa)(2)FyIP](ClO4)(2)center dot 2H(2)O (2) and [Ru(Hdpa)(2)IIP](ClO4)(2)center dot 2H(2)O (3) have been synthesized and characterized by spectroscopic techniques such as elemental analysis, UV/Vis, FT-IR, H-1 NMR, C-13 NMR and mass spectra. The CT-DNA binding properties of 1-3 have been investigated by absorption, emission spectroscopy and viscosity measurements. Experimental results suggested that they can interact with DNA through intercalative mode with different binding strengths. These were found to promote the cleavage of plasmid DNA. Cell viability results indicated that all compounds showed significant dose dependent cytotoxicity in selected cell lines and 1 shown higher cytotoxicity than dsplatin on HeLa cells. Cellular uptake studies were studied by flow cytometry and confocal microscopy. (C) 2013 Elsevier Masson SAS. All rights reserved.

引用

页码：160 / 169

页数：10

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