The protective effect of eicosapentaenoic acid-enriched phospholipids from sea cucumber Cucumaria frondosa on oxidative stress in PC12 cells and SAMP8 mice

被引:85
作者
Wu, Feng-Juan [1 ]
Xue, Yong [1 ]
Liu, Xiao-Fang [1 ]
Xue, Chang-Hu [1 ]
Wang, Jing-Feng [1 ]
Du, Lei [1 ]
Takahashi, Koretaro [2 ]
Wang, Yu-Ming [1 ]
机构
[1] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Shandong, Peoples R China
[2] Hokkaido Univ, Fac Fisheries Sci, Div Marine Life Sci, Hakodate, Hokkaido 0418611, Japan
基金
中国国家自然科学基金;
关键词
Alzheimer's disease; Oxidative stress; Eicosapentaenoic acid-enriched; phospholipids; Antioxidant; Learning and memory impairments; ACCELERATED MOUSE SAM; DOCOSAHEXAENOIC ACID; HIPPOCAMPUS; INVOLVEMENT; INJURY; MEMORY; BRAIN;
D O I
10.1016/j.neuint.2013.10.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alzheimer's disease (AD) is a common neurodegenerative disorders, in which oxidative stress plays an important role. The present study investigated the effect of eicosapentaenoic acid-enriched phospholipids (EPA-enriched PL) from the sea cucumber Cucumaria frondosa on oxidative injury in PC12 cells induced by hydrogen peroxide (H2O2) and tert-butylhydroperoxide (t-BHP). We also studied the effect of EPA-enriched PL on learning and memory functions in senescence-accelerated prone mouse strain 8 (SAMP8) in vivo. Pretreatment with EPA-enriched PL resulted in an enhancement of survival in a dose-dependent manner in H2O2 or t-BHP damaged PC12 cells. EPA-enriched PL pretreatment could also reduce the leakage of lactate dehydrogenase (LDH), and increase the intracellular total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity compared with the H2O2 or t-BHP group. The down-regulated Bcl-2 mRNA level and up-regulated Bax, Caspase-9, and Caspase-3 mRNA expression induced by H2O2 or t-BHP could be restored by EPA-enriched PL pretreatment. These results demonstrated that EPA-enriched PL exhibited its neuroprotective effects by virtue of its antioxidant activity, which might be achieved by inhibiting the mitochondria-dependent apoptotic pathway. The neuroprotective effect of EPA-enriched PL was also verified in vivo test: the EPA-enriched PL administration prevented the development of learning and memory impairments in SAMP8 mice. Our results indicated that EPA-enriched PL could offer an efficient and novel strategy to explore novel drugs or functional food for neuronprotection and cognitive improvement. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:9 / 17
页数:9
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