Aberrant expression of serpin squamous cell carcinoma antigen 2 in human tumor tissues and cell lines: Evidence of protection from tumor necrosis factor-mediated apoptosis

被引：24

作者：

Takeda, A

Kajiya, A

Iwasawa, A

Nakamura, Y

Hibino, T

机构：

[1] Sagami Womens Univ, Fac Arts & Sci, Biochem Lab, Sagamihara, Kanagawa 2288533, Japan

[2] Showa Univ, Fujigaoka Hosp, Dept Clin Pathol, Aoba Ku, Yokohama, Kanagawa 2278501, Japan

[3] Shiseido Res Ctr, Kanazawa Ku, Yokohama, Kanagawa 2368643, Japan

来源：

BIOLOGICAL CHEMISTRY | 2002年 / 383卷 / 7-8期

关键词：

apoptosis; RT-PCR; SCCAs; serpin; TNF-alpha;

D O I：

10.1515/BC.2002.136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Squamous cell carcinoma antigens (SCCA) 1 and 2 are highly homologous proteins of the serpin family, although they inhibit different types of proteinases. We investigated the expression of both SCCA mRNAs in tumor tissues, in various cell lines (A431, SAS, Ca9, HeLa, SKGIIIa, HSC-2, HSC-3, HSC-4 and KB) and in HSC cell lines in the presence of tumor necrosis factor alpha (TNFalpha). The expression of SCCA2 mRNA could be differentiated from that of SCCA1 in tumor tissues and cell lines by means of reverse transcriptionpolymerase chain reaction (RTPCR). The ratio between SCCA1 and SCCA2 mRNA expression showed selective expression of SCCA2 mRNA in SCC tissues from the uterine cervix compared to SCC tissues from the esophagus or skin. In addition, a significant level of SCCA2 mRNA expression was detected in the HSC-4 cell line, but not in Ca9, HeLa, SKGIIIa, or HSC-3 cells. In contrast, SCCA1 mRNA was detected in all samples tested. These results suggest that the level of expression of SCCA2 mRNA detected by RTPCR can be used to evaluate the status of SCC tumors. Next, we studied the effect of TNF-alpha on SCCA1 and SCCA2 mRNA expression in HSC cell lines. SCCA1 mRNA expression was constantly increased in the three HSC cells examined with increasing time of exposure to TNF-alpha. In contrast, SCCA2 mRNA expression was specific for HSC-4 cells. The survival rate of HSC-4 cells pretreated with TNF-alpha (6.3 ng/ml) for 48 h was found to be 72%, compared with 42% and 9% for HSC-3 and HSC-2 cells, respectively, after apoptotic stimulation by TNF-alpha (10 ng/ml) and cycloheximide (10 g/ml) for 18 h. Furthermore, selective expression of SCCA2 mRNA in HSC-4 pretreated with TNF-alpha protected these cells from TNF-alpha-mediated apoptosis. Thus, SCCA2 overexpression in squamous tumor cells contributed to their survival by protecting them against TNF-alpha-induced apoptosis.

引用

页码：1231 / 1236

页数：6

共 42 条

[1]

BAGLIONI C, 1985, J BIOL CHEM, V260, P3395

[2]

BEVILACQUA MP, 1986, P NATL ACAD SCI USA, V83, P5433

[3] Co-expression of the squamous cell carcinoma antigens 1 and 2 in normal adult human tissues and squamous cell carcinomas [J].

Cataltepe, S ;

Gornstein, ER ;

Schick, C ;

Kamachi, Y ;

Chatson, K ;

Fries, J ;

Silverman, GA ;

Upton, MP .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2000, 48 (01) :113-122

[4]

EAGLE H, 1958, P SOC EXP BIOL MED, V94, P661

[5]

ERCOLANI L, 1988, J BIOL CHEM, V263, P15335

[6] APOPTOSIS IN CANCER-THERAPY - CROSSING THE THRESHOLD [J].

FISHER, DE .

CELL, 1994, 78 (04) :539-542

[7] PREVENTION OF VERTEBRATE NEURONAL DEATH BY THE CRMA GENE [J].

GAGLIARDINI, V ;

FERNANDEZ, PA ;

LEE, RKK ;

DREXLER, HCA ;

ROTELLO, RJ ;

FISHMAN, MC ;

YUAN, J .

SCIENCE, 1994, 263 (5148) :826-828

[8]

GEY GO, 1952, CANCER RES, V12, P264

[9] IN-VITRO CULTIVATION OF HUMAN TUMORS - ESTABLISHMENT OF CELL LINES DERIVED FROM A SERIES OF SOLID TUMORS [J].

GIARD, DJ ;

AARONSON, SA ;

TODARO, GJ ;

ARNSTEIN, P ;

KERSEY, JH ;

DOSIK, H ;

PARKS, WP .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1973, 51 (05) :1417-1423

[10]

HORIKOSHI M, 1974, JPN J ORAL SURG, V2, P100

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