Microfluidic generation of droplet interface bilayer networks incorporating real-time size sorting in linear and non-linear configurations

被引：11

作者：

Carreras, P. ^{[1
]}

Law, R. V. ^{[1
]}

Brooks, N. ^{[1
]}

Seddon, J. M. ^{[1
]}

Ces, O. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

来源：

BIOMICROFLUIDICS | 2014年 / 8卷 / 05期

基金：

英国工程与自然科学研究理事会;

关键词：

LIPID-BILAYERS;

D O I：

10.1063/1.4897495

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a novel droplet based microfluidic method for the generation of different sized droplet interface bilayers is reported. A microfluidic platform was designed, which allows the generation and packing of picoliter lipid coated water droplets. Droplets were generated by hydrodynamic focusing coupled with selective transport along grooves according to their size. A trapping structure at the end of the groove and a fine control of the flow pressures allowed for the droplets to be successfully trapped and aligned on demand. This technology facilitates the fine control of droplet size production as well as the generation of extended networks from a variety of lipids including 1,2-diphytanoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphocholine in linear and nonlinear configurations, which is vital to the application of Droplet Interface Bilayers to biological network construction on-chip. (C) 2014 AIP Publishing LLC.

引用

页数：9

共 15 条

[1] Formation of artificial lipid bilayers using droplet dielectrophoresis [J].

Aghdaei, Sara ;

Sandison, Mairi E. ;

Zagnoni, Michele ;

Green, Nicolas G. ;

Morgan, Hywel .

LAB ON A CHIP, 2008, 8 (10) :1617-1620

[2] A double droplet trap system for studying mass transport across a droplet-droplet interface [J].

Bai, Yunpeng ;

He, Ximin ;

Liu, Dingsheng ;

Patil, Santoshkumar N. ;

Bratton, Dan ;

Huebner, Ansgar ;

Hollfelder, Florian ;

Abell, Chris ;

Huck, Wilhelm T. S. .

LAB ON A CHIP, 2010, 10 (10) :1281-1285

[3] Droplet interface bilayers [J].

Bayley, Hagan ;

Cronin, Brid ;

Heron, Andrew ;

Holden, Matthew A. ;

Hwang, William L. ;

Syeda, Ruhma ;

Thompson, James ;

Wallace, Mark .

MOLECULAR BIOSYSTEMS, 2008, 4 (12) :1191-1208

[4] Novel technologies for the formation of 2-D and 3-D droplet interface bilayer networks [J].

Elani, Yuval ;

deMello, Andrew J. ;

Niu, Xize ;

Ces, Oscar .

LAB ON A CHIP, 2012, 12 (18) :3514-3520

[5] Lipid bilayer formation by contacting monolayers in a microfluidic device for membrane protein analysis [J].

Funakoshi, Kei ;

Suzuki, Hiroaki ;

Takeuchi, Shoji .

ANALYTICAL CHEMISTRY, 2006, 78 (24) :8169-8174

[6] Functional bionetworks from nanoliter water droplets [J].

Holden, Matthew A. ;

Needham, David ;

Bayley, Hagan .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (27) :8650-8655

[7] Asymmetric droplet interface bilayers [J].

Hwang, William L. ;

Chen, Min ;

Cronin, Brid ;

Holden, Matthew A. ;

Bayley, Hagan .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (18) :5878-+

[8] Electrical behavior of droplet interface bilayer networks: Experimental analysis and Modeling [J].

Hwang, William L. ;

Holden, Matthew A. ;

White, Steven ;

Bayley, Hagan .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (38) :11854-11864

[9] Interdroplet bilayer arrays in millifluidic droplet traps from 3D-printed moulds [J].

King, Philip H. ;

Jones, Gareth ;

Morgan, Hywel ;

de Planque, Maurits R. R. ;

Zauner, Klaus-Peter .

LAB ON A CHIP, 2014, 14 (04) :722-729

[10]

Maglia G, 2009, NAT NANOTECHNOL, V4, P437, DOI [10.1038/nnano.2009.121, 10.1038/NNANO.2009.121]

← 1 2 →