In situ synthesized rGO-Fe3O4 nanocomposites as enzyme immobilization support for achieving high activity recovery and easy recycling

被引：55

作者：

Yang, Dong ^{[1
,2
]}

Wang, Xueyan ^{[1
,3
]}

Shi, Jiafu ^{[2
,3
]}

Wang, Xiaoli ^{[1
,3
]}

Zhang, Shaohua ^{[1
,3
]}

Han, Pingping ^{[1
,3
]}

Jiang, Zhongyi ^{[1
,3
]}

机构：

[1] Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Green Chem Technol,Key Lab Bioengn, Tianjin 300072, Peoples R China

[2] Tianjin Univ, Sch Environm Sci & Engn, Tianjin 300072, Peoples R China

[3] Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2016年 / 105卷

关键词：

rGO-Fe3O4; nanocomposites; Immobilised enzyme; Enzyme activity; Easy recycling; Kinetic parameters; Biocatalysis; GRAPHENE OXIDE; MAGNETIC NANOPARTICLES; GRAPHITE OXIDE; RAMAN-SPECTRA; FUNCTIONALIZATION; ADSORPTION; CATALASE; AEROGELS; SHEETS;

D O I：

10.1016/j.bej.2015.10.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Herein, the reduced graphene oxide-Fe3O4 (rGO-Fe3O4) nanocomposites are synthesized by the simultaneous reduction of graphene oxide (GO) and in situ deposition of Fe3O4 nanoparticles (ca. 20 nm) enabled by Fe2+ ions. The rGO-Fe3O4 nanocomposites integrate the magnetic property of Fe3O4 nanoparticles and the large specific surface area of rGO nanosheets. Catalase (CAT), as a commonly used enzyme, can be efficiently immobilized on the rGO-Fe3O4 nanocomposites through physical adsorption. The CAT loading capacity is as high as 312.5 +/- 12.6 mg g(-1), while the activity recovery of CAT can be high up to nearly 98%. The strong magnetic response of immobilized CAT ensures its easy separation from the reaction system when an external magnetic field is applied. Owing to the hydrophobic and hydrogen bonding interactions between enzyme and the support, the immobilized CAT exhibits zero leaching, desirable stability and excellent reusability. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：273 / 280

页数：8

共 49 条

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