Multilevel algorithms and evolutionary hybrid tools for enhanced production of arginine deiminase from Pseudomonas furukawaii RS3

被引：18

作者：

Dhankhar, Rakhi ^{[1
]}

Kumar, Ashwani ^{[2
]}

Kumar, Sanjay ^{[1
]}

Chhabra, Deepak ^{[2
]}

Shukla, Pratyoosh ^{[3
]}

Gulati, Pooja ^{[1
]}

机构：

[1] Maharshi Dayanand Univ, Dept Microbiol, Med Microbiol & Bioproc Technol Lab, Rohtak, Haryana, India

[2] Maharshi Dayanand Univ, Univ Inst Engn & Technol, Dept Mech Engn, Optimizat & Mechatron Lab, Rohtak, Haryana, India

[3] Maharshi Dayanand Univ, Dept Microbiol, Enzyme Technol & Prot Bioinformat Lab, Rohtak 124001, Haryana, India

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 290卷

关键词：

Arginine deiminase; Anti-cancer enzyme; GA-ANN; GA-ANFIS; Purification; TUMOR-CELLS; PURIFICATION; ENZYMES; PATHWAY;

D O I：

10.1016/j.biortech.2019.121789

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In the present study a high arginine deiminase (ADI) yielding bacterium was isolated from soil samples of Haryana, India and identified as Pseudomonas furukawaii. The specific enzyme activity was optimized to 1.420 IU/ml by OFAT and further enhanced to 2.708 IU/ml (an increase of 90.7%) with the help of statistical parametric optimization approaches using GA-ANN and GA-ANFIS. The obtained value of the coefficient of correlation (R = 0.88) for ANN and epoch error (0.12) for ANFIS, indicates the prediction accuracy and strength of these data training models. ADI production was improved significantly in simple super broth media supplemented with 1.5% fructose and 1.75% arginine at pH 7 at 37 degrees C using multilevel algorithms and evolutionary hybrid tools. The native enzyme was partially purified (ten-fold) up to a specific enzyme activity of 29.559 IU/mg.

引用

页数：9

共 30 条

[1] Arginine catabolism by sourdough lactic acid bacteria:: Purification and characterization of the arginine deiminase pathway enzymes from Lactobacillus sanfranciscensis CB1 [J].