Long-PCR based next generation sequencing of the whole mitochondrial genome of the peacock skate Pavoraja nitida (Elasmobranchii: Arhynchobatidae)

被引：4

作者：

Yang, Lei ^{[1
]}

Naylor, Gavin J. P. ^{[1
]}

机构：

[1] Coll Charleston, Dept Biol, Charleston, SC 29401 USA

来源：

MITOCHONDRIAL DNA PART A | 2016年 / 27卷 / 02期

基金：

美国国家科学基金会;

关键词：

Annotation; chondrichthyes; long-PCR primers; mitogenome; NGS; Rajiformes;

D O I：

10.3109/19401736.2014.926497

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We determined the complete mitochondrial genome sequence (16,760bp) of the peacock skate Pavoraja nitida using a long-PCR based next generation sequencing method. It has 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region in the typical vertebrate arrangement. Primers, protocols, and procedures used to obtain this mitogenome are provided. We anticipate that this approach will facilitate rapid collection of mitogenome sequences for studies on phylogenetic relationships, population genetics, and conservation of cartilaginous fishes.

引用

页码：943 / 944

页数：2

共 4 条

[1] Method for improving sequence coverage uniformity of targeted genomic intervals amplified by LR-PCR using Illumina GA sequencing-by-synthesis technology [J].