Clinical potential of microchip capillary electrophoresis systems

被引：135

作者：

Colyer, CL ^{[1
]}

Tang, T ^{[1
]}

Chiem, N ^{[1
]}

Harrison, DJ ^{[1
]}

机构：

[1] UNIV ALBERTA, DEPT CHEM, EDMONTON, AB T6G 262, CANADA

来源：

ELECTROPHORESIS | 1997年 / 18卷 / 10期

关键词：

serum immunoassay; DNA analysis; microfluidics;

D O I：

10.1002/elps.1150181006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Clinical interest in the use of capillary electrophoresis (CE) has recently been extended to the microchip environment. Clinical analyses demand careful handling of complex samples that are often limited in quantity and in concentration. The integrated sample handling and analysis capabilities of microchip substrates thus seem ideally suited to clinical applications. This review surveys the development of sample handling (injection, mixing, and reaction) and separation elements on-chip. The integration of these elements to create a variety of clinical analyzers has been demonstrated. The application of microchip CE systems to human serum protein analysis, immunoassay, and DNA studies is reviewed, along with various other clinical applications. In addition, the clinical potential of the lab-on-a-chip concept is discussed.

引用

页码：1733 / 1741

页数：9

共 72 条

[1]

ALBIN M, 1996, 1996 SOL STAT SENS A, P253

[2] HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS FOR THE DETERMINATION OF TRYPSIN AND CHYMOTRYPSIN INHIBITORS AND THEIR ASSOCIATION WITH TRYPSIN, CHYMOTRYPSIN AND MONOCLONAL-ANTIBODIES [J].