Role of the stromal cell derived factor-1 in the biological functions of endothelial progenitor cells and its underlying mechanisms

被引:33
作者
Cun, Yanping [1 ]
Diao, Bo [2 ]
Zhang, Zhimin [1 ]
Wang, Gang [2 ]
Yu, Jing [2 ]
Ma, Lianting [3 ]
Rao, Zhiguo [1 ]
机构
[1] Gen Hosp Cent Theater Command, Dept Oncol, 627 Wuluo Rd, Wuhan 430070, Hubei, Peoples R China
[2] Gen Hosp Cent Theater Command, Dept Clin Expt, Wuhan 430070, Hubei, Peoples R China
[3] Gen Hosp Cent Theater Command, Dept Neurosurg, 627 Wuluo Rd, Wuhan 430070, Hubei, Peoples R China
基金
中国博士后科学基金;
关键词
endothelial progenitor cells; stromal cell derived factor-1; biological functions; Akt signaling pathway; ERK signaling pathway; CHEMOKINE RECEPTOR; VASCULAR REPAIR; GENE-TRANSFER; TUMOR-GROWTH; CXCR4; MIGRATION; FACTOR-1-ALPHA; CANCER; SDF-1/CXCL12; CONTRIBUTES;
D O I
10.3892/etm.2020.9471
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Stromal cell derived factor-1 (SDF-1) is a chemokine that plays a critical role in the homing of stem and progenitor cells, including endothelial progenitor cells (EPCs). However, little research has been undertaken to evaluate the roles of SDF-1 in the biological functions of EPCs and related signaling pathways. The present study aimed to investigate the biological functions of EPCs in response to SDF-1, as well as the underlying mechanisms. The effects of SDF-1 treatment on EPC proliferation, migration and tube formation were assessed by performing MTS, Transwell and in vitro tube formation assays, respectively. The phosphorylation status of Akt and ERK was evaluated by western blotting. The present results indicated that SDF-1 treatment enhanced EPC proliferation, migration and tube formation compared with the control group. Furthermore, SDF-1-induced EPC proliferation was significantly reduced following treatment with a C-X-C Motif Chemokine Receptor 4 antagonist (AMD3100), a PI3K inhibitor (LY294002) and the mitogen-activated protein kinase kinase inhibitor (MEK; PD98059). SDF-1-induced migration and angiogenesis were significantly suppressed by the PI3K inhibitor, but not the MEK inhibitor. Moreover, SDF-1 significantly increased the protein expression levels of phosphorylated (p)-Akt and p-ERK; however, SDF-1-induced effects on protein expression were suppressed by AMD3100, LY294002 and PD98059. Thus, SDF-1-induced EPC proliferation was mediated by activation of the Akt and ERK signaling pathways, whereas SDF-1-mediated EPC migration and tube formation only involved activation of the Akt signaling pathway.
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页数:9
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