Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd

被引：3

作者：

Hatami, Tayyebe ^{[1
,2
]}

Emami, Sayyed Ahmad ^{[3
]}

Miraghaee, Sayyed Shahram ^{[1
]}

Mojarrab, Mahdi ^{[1
]}

机构：

[1] Kermanshah Univ Med Sci, Sch Pharm, Novel Drug Delivery Res Ctr, Kermanshah, Iran

[2] Kermanshah Univ Med Sci, Student Res Comm, Kermanshah, Iran

[3] Mashhad Univ Med Sci, Sch Pharm, Dept Pharmacognosy, Mashhad, Iran

来源：

IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH | 2014年 / 13卷 / 02期

关键词：

Artemisia biennis; Total phenolic content; Antioxidant activity; METHANOLIC EXTRACT; ESSENTIAL OIL; FRUIT; L; POLYPHENOLS; METALS;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Total phenolic contents (TPC) of five different extracts (petroleum ether, dichloromethane, ethyl acetate, ethanol and ethanol-water) of Artemisia biennis Willd were measured in this work. The antioxidant activity was investigated by three different methods: beta-carotene bleaching (BCB) test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferrous ion chelating (FIC) assay. Among all the extracts analyzed, the hydroethanolic extract exhibited a significantly higher phenolic content and antioxidant activity than other samples. Vacuum liquid chromatography of this extract yielded seven fractions (A to G) which were subjected to all aforementioned experiments. The highest total phenolic content and free radical scavenging activites were present in the same sample (Fraction D) but the only statistically significant correlation between TPC and EC50 values was observed for BCB.

引用

页码：551 / 558

页数：8

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