ccSOL omics: a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli

被引：81

作者：

Agostini, Federico ^{[1
,2
]}

Cirillo, Davide ^{[1
,2
]}

Maria Livi, Carmen ^{[1
,2
]}

Delli Ponti, Riccardo ^{[1
,2
]}

Gaetano Tartaglia, Gian ^{[1
,2
]}

机构：

[1] Ctr Genom Regulat CRG, Barcelona 08003, Spain

[2] UPF, Barcelona 08003, Spain

来源：

BIOINFORMATICS | 2014年 / 30卷 / 20期

基金：

欧洲研究理事会;

关键词：

PROTEIN SOLUBILITY; AGGREGATION; SEQUENCES;

D O I：

10.1093/bioinformatics/btu420

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A Summary: Here we introduce ccSOL omics, a webserver for largescale calculations of protein solubility. Our method allows (i) proteome-wide predictions; (ii) identification of soluble fragments within each sequences; (iii) exhaustive single-point mutation analysis. Results: Using coil/disorder, hydrophobicity, hydrophilicity, beta-sheet and alpha-helix propensities, we built a predictor of protein solubility. Our approach shows an accuracy of 79% on the training set (36990 Target Track entries). Validation on three independent sets indicates that ccSOL omics discriminates soluble and insoluble proteins with an accuracy of 74% on 31760 proteins sharing <30% sequence similarity.

引用

页码：2975 / 2977

页数：3

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