Influence of age on rat bone-marrow mesenchymal stem cells potential

被引：54

作者：

Fafian-Labora, J. ^{[1
]}

Fernandez-Pernas, P. ^{[1
]}

Fuentes, I. ^{[1
]}

De Toro, J. ^{[1
]}

Oreiro, N. ^{[2
]}

Sangiao-Alvarellos, S. ^{[3
]}

Mateos, J. ^{[1
]}

Arufe, M. C. ^{[1
]}

机构：

[1] Univ A Coruna, Grp Terapia Celular Med Regenerat TCMR CHUAC, CIBER BBN ISCIII,CHUAC,Dept Med,Fac Oza, Serv Reumatol,Inst Invest Biomed A Coruna INIBIC, As Xubias 15006, A Coruna, Spain

[2] Univ A Coruna, Grp Prote ProteoRed ISCIII PBR2, Serv Reumatol,CHUAC, Inst Invest Biomed A Coruna INIBIC, As Xubias 15006, A Coruna, Spain

[3] Univ A Coruna, Grp Fisiopatol Endocrina Nutr & Med FENM CHUAC, Inst Invest Biomed A Coruna INIBIC, CHUAC,Dept Med,Fac Oza, As Xubias 15006, A Coruna, Spain

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

CELLULAR PROLIFERATION; DIFFERENTIATION; SENESCENCE; EXPRESSION; PLURIPOTENCY; IMPAIRMENT; ACTIVATION; MIGRATION; PROTEIN; TISSUE;

D O I：

10.1038/srep16765

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mesenchymal stem cells promising role in cell-based therapies and tissue engineering appears to be limited due to a decline of their regenerative potential with increasing donor age. Six age groups from bone marrow mesenchymal stem cells of Wistar rats were studied (newborn, infant, young, pre-pubertal, pubertal and adult). Quantitative proteomic assay was performance by iTRAQ using an 8-plex iTRAQ labeling and the proteins differentially expressed were grouped in pluripotency, proliferative and metabolism processes. Proliferation makers, CD117 and Ki67 were measure by flow cytometry assay. Real time polymerase chain reaction analysis of pluripotency markers Rexl, Oct4, Sox2 and Nanog were done. Biological differentiation was realized using specific mediums for 14 days to induce osteogenesis, adipogenesis or chondrogenesis and immunostain analysis of differentiated cell resulting were done. Enzimoimmunoassay analysis of several enzymes as L-lactate dehydrogenase and glucose-6-phosphate isomerase were also done to validate iTRAQ data. Taking together these results indicate for the first time that mesenchymal stem cells have significant differences in their proliferative, pluripotency and metabolism profiles and those differences are age depending.

引用

页数：20

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