New Cytotoxic Steroid Produced by the Soil-Derived Fungus Aspergillus flavus JDW-1

One new steroid 1, together with seven known compounds 2 to 8, were discovered in the extract of a soil-derived fungus Aspergillus flavus JDW-1. The structure, including the absolute stereochemistry of new compound 1, was established by interpretation of extensive nuclear magnetic resonance spectroscopic data and further confirmed by X-ray crystallographic analysis. The cytotoxicity of 1 against the A-549, Hela, HCT-116, MGC-803, and HO-8910 cell lines was evaluated, which showed cytotoxicity with the half-maximal inhibitory concentration (IC50) values as 5.00 to 22.38 M. Compounds 2 to 8 exhibited moderate radical scavenging activity against DPPH with IC50 values ranging from 4.7 to 28.5 M.