Effects of polysaccharides from Pholiota nameko on maturation of murine bone marrow-derived dendritic cells

被引：16

作者：

Li, Haiping ^{[1
]}

Liu, Lizeng ^{[1
]}

Tao, Yongqing ^{[1
]}

Zhao, Pei ^{[1
]}

Wang, Fengling ^{[1
]}

Huai, Lihua ^{[1
]}

Zhi, Dexian ^{[1
]}

Liu, Jiangmei ^{[1
]}

Li, Guoliang ^{[1
]}

Dang, Chunlan ^{[1
]}

Xu, Yufeng ^{[1
]}

机构：

[1] Tianjin Univ Commerce, Fac Biotechnol & Food Sci, Tianjin Key Lab Food Biotechnol, Tianjin 300134, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2014年 / 63卷

基金：

中国国家自然科学基金;

关键词：

Pholiota Nameko; Polysaccharide; Dendritic cells; Maturation; Immunomodulation; ANTIOXIDANT ACTIVITIES; STRUCTURAL-CHARACTERIZATION; ACIDIC POLYSACCHARIDES; GRIFOLA-FRONDOSA; LARGE NUMBERS; GENERATION; EXTRACTION; MOLECULES;

D O I：

10.1016/j.ijbiomac.2013.11.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper studied some structure characters of the Pholiota nameko polysaccharides (PNPS-1), including morphology under SEM and AFM, also the effects of PNPS-1 on the maturation of bone marrow dendritic cells (BMDCs) via concrete changes both inside and outside BMDCs. These impacts on BMDCs were assessed with use of inverted phase contrast microscope for morphology, flow cytometry for key surface molecules, mixed lymphocyte reaction (MLR) for allogeneic T cells proliferation, and bio-assay and enzyme linked immunosorbent assay (ELISA) for cytokine production. We found that PNPS-1 could inhibit phenotypic maturation as evidenced by decreasing expression of CD11c, CD40, CD80, CD83, CD86, and I-A/I-E. Functional maturation inhibition was further confirmed by decreased naive T cell stimulatory activity of BMDCs. Finally, PNPS-1 also stimulated production of more cytokine IL-10 and less IL-12 and TNF-alpha. These data indicated that PNPS-1 could markedly inhibit the maturation of BMDCs and had potential significant down-regulation immunity. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：188 / 197

页数：10

共 60 条

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