Dihydroartemisinin alleviates oxidative stress in bleomycin-induced pulmonary fibrosis

被引：58

作者：

Yang, Dong-xia ^{[1
]}

Qiu, Jun ^{[2
]}

Zhou, Hui-Hui ^{[3
]}

Yu, Yan ^{[1
]}

Zhou, Dong-li ^{[4
]}

Xu, Yan ^{[5
]}

Zhu, Ming-zhe ^{[5
]}

Ge, Xing-ping ^{[5
]}

Li, Jing-min ^{[1
]}

Lv, Chang-jun ^{[1
]}

Zhang, Hong-qin ^{[1
]}

Yuan, Wen-dan ^{[1
]}

机构：

[1] Binzhou Med Univ, Coll Basic Med, Yantai 264003, Shandong, Peoples R China

[2] Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Blood Purificat Ctr, Yantai 264000, Shandong, Peoples R China

[3] Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Pathol Ctr, Yantai 264000, Shandong, Peoples R China

[4] Shandong Laiyang Hlth Sch, Laiyang 265200, Shandong, Peoples R China

[5] Binzhou Med Univ, Affiliated Hosp, Pediat Nephro Dept, Yantai 264000, Shandong, Peoples R China

来源：

LIFE SCIENCES | 2018年 / 205卷

基金：

中国国家自然科学基金;

关键词：

Dihydroartemisinin; Oxidative stress; Bleomycin; Pulmonary fibrosis; LOWER RESPIRATORY-TRACT; GLUTATHIONE DEFICIENCY; NAD(P)H OXIDASE; LUNG FIBROSIS; ACTIVATION; ALPHA; PATHOGENESIS; CELLS; MICE;

D O I：

10.1016/j.lfs.2018.05.022

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aims: Dihydroartemisinin has been shown to inhibit the development of pulmonary fibrosis in rats, but its mechanism has yet to be elucidated. This study aimed to determine the mechanisms of dihydroartemisinin in bleomycin-induced pulmonary fibrosis in a rat model. Main methods: Morphological changes and collagen deposition were analyzed via hematoxylin-eosin staining and Masson staining and the expression of biotic-factor-related oxidative stress in lung tissues was assayed with standard assay kits. The expressions of alpha-SMA, E-cadherin, and Nrf2/HO-1 were detected by Western blot and RT-PCR, and the cell morphology and proliferation of cultured type II alveolar epithelial cells (AECs) were assessed via microscopy and immunocytochemical assay. Key findings: Dihydroartemisinin treatment significantly decreased the level of oxidative stress and collagen synthesis and inhibited AECs differentiation in bleomycin-induced pulmonary fibrosis compared to the control group (P < 0.001). Significance: Our results indicated that dihydroartemisinin might decrease oxidative damage to attenuate lung injury and fibrosis.

引用

页码：176 / 183

页数：8

共 38 条

[1] Protective Effect of Infliximab, a Tumor Necrosis Factor-Alfa Inhibitor, on Bleomycin-Induced Lung Fibrosis in Rats [J].

Altintas, Nejat ;

Erboga, Mustafa ;

Aktas, Cevat ;

Bilir, Bulent ;

Aydin, Murat ;

Sengul, Aysun ;

Ates, Zehra ;

Topcu, Birol ;

Gurel, Ahmet .

INFLAMMATION, 2016, 39 (01) :65-78

[2] Oxidative stress in the pathogenesis of diffuse lung diseases: A review [J].

Bargagli, E. ;

Olivieri, C. ;

Bennett, D. ;

Prasse, A. ;

Muller-Quernheim, J. ;

Rottoli, P. .

RESPIRATORY MEDICINE, 2009, 103 (09) :1245-1256

[3] Glutathione deficiency of the lower respiratory tract in patients with idiopathic pulmonary fibrosis [J].

Beeh, KM ;

Beier, J ;

Haas, IC ;

Kornmann, O ;

Micke, P ;

Buhl, R .

EUROPEAN RESPIRATORY JOURNAL, 2002, 19 (06) :1119-1123

[4] NAD(P)H Oxidase Mediates TGF-β1-Induced Activation of Kidney Myofibroblasts [J].

Bondi, Corry D. ;

Manickam, Nagaraj ;

Lee, Duck Yoon ;

Block, Karen ;

Gorin, Yves ;

Abboud, Hanna E. ;

Barnes, Jeffrey L. .

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2010, 21 (01) :93-102

[5] GLUTATHIONE DEFICIENCY IN THE EPITHELIAL LINING FLUID OF THE LOWER RESPIRATORY-TRACT IN IDIOPATHIC PULMONARY FIBROSIS [J].

CANTIN, AM ;

HUBBARD, RC ;

CRYSTAL, RG .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1989, 139 (02) :370-372

[6] Cigarette Smoke Enhances the Expression of Profibrotic Molecules in Alveolar Epithelial Cells [J].

Checa, Marco ;

Hagood, James S. ;

Velazquez-Cruz, Rafael ;

Ruiz, Victor ;

Garcia-De-Alba, Carolina ;

Rangel-Escareno, Claudia ;

Urrea, Francisco ;

Becerril, Carina ;

Montano, Martha ;

Garcia-Trejo, Semiramis ;

Cisneros Lira, Jose ;

Aquino-Galvez, Arnoldo ;

Pardo, Annie ;

Selman, Moises .

PLOS ONE, 2016, 11 (03)

[7] Dihydroartemisinin alleviates bile duct ligation-induced liver fibrosis and hepatic stellate cell activation by interfering with the PDGF-βR/ERK signaling pathway [J].

Chen, Qin ;

Chen, Lianyun ;

Kong, Desong ;

Shao, Jiangjuan ;

Wu, Li ;

Zheng, Shizhong .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2016, 34 :250-258

[8] Cadherins and Epithelial-to-Mesenchymal Transition [J].

Gheldof, Alexander ;

Berx, Geert .

MOLECULAR BIOLOGY OF CADHERINS, 2013, 116 :317-336

[9] IPF: new insight on pathogenesis and treatment [J].

Harari, S. ;

Caminati, A. .

ALLERGY, 2010, 65 (05) :537-553

[10] Preventive Effects of Pomegranate Seed Extract on Bleomycin-Induced Pulmonary Fibrosis in Rat [J].

Hemmati, Ali Asghar ;

Rezaie, Annahita ;

Darabpour, Pegah .

JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS, 2013, 8 (02) :76-80

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