A triple-amplification strategy based on the formation of peroxidase-like two-dimensional DNA/Fe3O4 networks initiated by the hybridization chain reaction for highly sensitive detection of microRNA

被引：29

作者：

Tang, Sheng ^{[1
]}

Li, Yana ^{[1
]}

Zhu, Anni ^{[1
]}

Yao, Yao ^{[1
]}

Sun, Jun ^{[1
]}

Zheng, Fenfen ^{[1
]}

Lin, Zixia ^{[2
]}

Shen, Wei ^{[1
]}

机构：

[1] Jiangsu Univ Sci & Technol, Sch Environm & Chem Engn, Zhergiang 212003, Jiangsu, Peoples R China

[2] Yangzhou Univ, Testing Ctr, Yangzhou 225000, Jiangsu, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 58期

基金：

中国国家自然科学基金;

关键词：

SIGNAL AMPLIFICATION; IDENTIFICATION; NANOPARTICLES; EXPRESSION;

D O I：

10.1039/c9cc03194d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A highly sensitive triple-amplification assay for the detection of microRNA (miRNA) let-7a is reported in this work. The assay relies on the formation of magnetic two-dimensional DNA/Fe3O4 nanosheet networks initiated by the target miRNA-associated hybridization chain reaction. The Fe3O4 nanosheets in the DNA/Fe3O4 networks display peroxidase-like catalytic activity towards a colorimetric reaction, thereby producing a highly sensitive signal for the quantification of let-7a. Under optimal conditions, the assay achieved a detection limit of 13 aM at a signal-to-noise ratio of 3 and a linear calibration plot between 0.05 fM and 12 nM. Successful attempts were made in the quantification of let-7a in serum samples.

引用

页码：8386 / 8389