Electron capture dissociation in a radio frequency ion trap

被引：84

作者：

Baba, T ^{[1
]}

Hashimoto, Y ^{[1
]}

Hasegawa, H ^{[1
]}

Hirabayashi, A ^{[1
]}

Waki, I ^{[1
]}

机构：

[1] Hitachi Ltd, Cent Res Lab, Tokyo 1858601, Japan

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 15期

关键词：

D O I：

10.1021/ac049309h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report on the first evidence of electron capture dissociation (ECD) in a radio frequency (rf) ion trap. Peptide ions, [substance P](2+), trapped in a two-dimensional, linear rf ion trap were cleaved by electrons injected along the central axis of the trap. Along the axis, the if field component was zero and a magnetic field of 50 mT was applied. Ibis electron injection scheme keeps the energy of the electrons below 1 eV, preventing them from heating by the rf field. The present ECD efficiency is similar to4% by irradiation of electron current of 0.2 muA for 80 ms. ECD in rf traps may open high-throughput and low-cost ECD applications to obtain molecular structure information complementary to collision-induced dissociation.

引用

页码：4263 / 4266

页数：4

共 7 条

[1]

BABA T, 2003, P 51 ASMS C MASS SPE

[2]

IVONIN IA, 2003, P 51 ASMS C MASS SPE

[3] Dissociative capture of hot (3-13 eV) electrons by polypeptide polycations: an efficient process accompanied by secondary fragmentation [J].