Derivatization, separation and determination of steroids and triterpenoids by reverse-phase HPLC and UV detection

被引：2

作者：

Wang, SP

Lai, JS

Lin, ST

机构：

[1] Department of Applied Chemistry, Providence University, Shalu

来源：

JOURNAL OF THE CHINESE CHEMICAL SOCIETY | 1997年 / 44卷 / 04期

关键词：

steroids; triterpenoids; benzoate derivatives; HPLC; GLC;

D O I：

10.1002/jccs.199700059

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The steroids were derivatised with aroyl chloride to yield the corresponding benzoates. Among them, their 4-methoxybenzoates were obtained in greatest yield and were analyzed by HPLC with the smallest detection limit (10 ng) by means of a UV detector (254 nm). This method of derivatization can be applied for analysis of steroids and triterpenoids from crude products with the HPLC technique.

引用

页码：395 / 399

页数：5

共 8 条

[1] DETERMINATION OF STEROL AND TRITERPENE ALCOHOL ACETATES IN NATURAL-PRODUCTS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
BIANCHINI, JP
GAYDOU, EM
SIGOILLOT, JC
TERROM, G
[J]. JOURNAL OF CHROMATOGRAPHY, 1985, 329 (02): : 231 - 246
[2] SEPARATION OF BIOACTIVE QUADRI-TERPENIC ACIDS FROM THE FRUIT OF LIGUSTRUM-LUCIDUM AIT BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
DU, QZ
XIONG, XP
ITO, Y
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1995, 18 (10): : 1997 - 2004
[3] FURNISS BS, 1978, VOGELS TXB PRACTICAL, P838
[4] HEFTMANN E, 1983, CHROMATOGRAPHY, pA14
[5] IKAN R, 1991, NATURAL PRODUCTS, P145
[6] STRUCTURE-RETENTION RELATIONSHIP OF STEROLS AND TRITERPENE ALCOHOLS IN GAS-CHROMATOGRAPHY ON A GLASS-CAPILLARY COLUMN
ITOH, T
TANI, H
FUKUSHIMA, K
TAMURA, T
MATSUMOTO, T
[J]. JOURNAL OF CHROMATOGRAPHY, 1982, 234 (01): : 65 - 76
[7] SCUDDER PH, 1992, ELECT FLOW ORGANIC C, P72
[8] SNYDER LR, 1979, INTRO MODERN LIQUID, P165

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