Microbiome Patterns in Matched Bile, Duodenal, Pancreatic Tumor Tissue, Drainage, and Stool Samples: Association with Preoperative Stenting and Postoperative Pancreatic Fistula Development

被引:19
作者
Langheinrich, Melanie [1 ]
Wirtz, Stefan [2 ]
Kneis, Barbara [1 ]
Gittler, Matthias M. [1 ]
Tyc, Olaf [3 ]
Schierwagen, Robert [3 ]
Brunner, Maximilian [1 ]
Krautz, Christian [1 ]
Weber, Georg F. [1 ]
Pilarsky, Christian [1 ]
Trebicka, Jonel [3 ]
Agaimy, Abbas [4 ]
Grutzmann, Robert [1 ]
Kersting, Stephan [1 ]
机构
[1] Univ Hosp Erlangen, Dept Surg, D-91054 Erlangen, Germany
[2] Univ Hosp Erlangen, Dept Internal Med 1, D-91054 Erlangen, Germany
[3] Univ Clin Frankfurt, Dept Internal Med 1, Translat Hepatol, D-60590 Frankfurt, Germany
[4] Univ Hosp Erlangen, Dept Pathol, D-91054 Erlangen, Germany
关键词
microbiome; pancreatic surgery; postoperative complications; pancreatic cancer; 16S rRNA sequencing; RISK SCORE; CANCER;
D O I
10.3390/jcm9092785
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Postoperative complications after pancreatic surgery are still a significant problem in clinical practice. The aim of this study was to characterize and compare the microbiomes of different body compartments (bile duct, duodenal mucosa, pancreatic tumor lesion, postoperative drainage fluid, and stool samples; preoperative and postoperative) in patients undergoing pancreatic surgery for suspected pancreatic cancer, and their association with relevant clinical factors (stent placement, pancreatic fistula, and gland texture). For this, solid (duodenal mucosa, pancreatic tumor tissue, stool) and liquid (bile, drainage fluid) biopsy samples of 10 patients were analyzed using 16s rRNA gene next-generation sequencing. Our analysis revealed: (i) a distinct microbiome in the different compartments, (ii) markedly higher abundance of Enterococcus in patients undergoing preoperative stent placement in the common bile duct, (iii) significant differences in the beta diversity between patients who developed a postoperative pancreatic fistula (POPF B/C), (iv) patients with POPF B/C were more likely to have bacteria belonging to the genus Enterococcus, and (v) differences in microbiome composition with regard to the pancreatic gland texture. The structure of the microbiome is distinctive in different compartments, and can be associated with the development of a postoperative pancreatic fistula.
引用
收藏
页码:1 / 17
页数:17
相关论文
共 38 条
[21]   Infection After Pancreatic Surgery Superficial and Deep Thoughts [J].
Matthews, Jeffrey B. .
JAMA SURGERY, 2017, 152 (11) :1030-1030
[22]   The Tumor Microbiome in Pancreatic Cancer: Bacteria and Beyond [J].
McAllister, Florencia ;
Khan, Md Abdul Wadud ;
Helmink, Beth ;
Wargo, Jennifer A. .
CANCER CELL, 2019, 36 (06) :577-579
[23]   A Multi-Institutional External Validation of the Fistula Risk Score for Pancreatoduodenectomy [J].
Miller, Benjamin C. ;
Christein, John D. ;
Behrman, Stephen W. ;
Drebin, Jeffrey A. ;
Pratt, Wande B. ;
Callery, Mark P. ;
Vollmer, Charles M., Jr. .
JOURNAL OF GASTROINTESTINAL SURGERY, 2014, 18 (01) :172-179
[24]   Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia [J].
Pereira, Pedro ;
Aho, Velma ;
Arola, Johanna ;
Boyd, Sonja ;
Jokelainen, Kalle ;
Paulin, Lars ;
Auvinen, Petri ;
Farkkila, Martti .
PLOS ONE, 2017, 12 (08)
[25]   Risk prediction for development of pancreatic fistula using the ISGPF classification scheme [J].
Pratt, Wande B. ;
Callery, Mark P. ;
Vollmer, Charles M., Jr. .
WORLD JOURNAL OF SURGERY, 2008, 32 (03) :419-428
[26]   The Pancreatic Cancer Microbiome Promotes Oncogenesis by Induction of Innate and Adaptive Immune Suppression [J].
Pushalkar, Smruti ;
Hundeyin, Mautin ;
Daley, Donnele ;
Zambirinis, Constantinos P. ;
Kurz, Emma ;
Mishra, Ankita ;
Mohan, Navyatha ;
Aykut, Berk ;
Usyk, Mykhaylo ;
Torres, Luisana E. ;
Werba, Gregor ;
Zhang, Kevin ;
Guo, Yuqi ;
Li, Qianhao ;
Akkad, Neha ;
Lall, Sarah ;
Wadowski, Benjamin ;
Gutierrez, Johana ;
Rossi, Juan Andres Kochen ;
Herzog, Jeremy W. ;
Diskin, Brian ;
Torres-Hernandez, Alejandro ;
Leinwand, Josh ;
Wang, Wei ;
Taunk, Pardeep S. ;
Savadkar, Shivraj ;
Janal, Malvin ;
Saxena, Anjana ;
Li, Xin ;
Cohen, Deirdre ;
Sartor, R. Balfour ;
Saxena, Deepak ;
Miller, George .
CANCER DISCOVERY, 2018, 8 (04) :403-416
[27]   Tumor Microbiome Diversity and Composition Influence Pancreatic Cancer Outcomes [J].
Riquelme, Erick ;
Zhang, Yu ;
Zhang, Liangliang ;
Montiel, Maria ;
Zoltan, Michelle ;
Dong, Wenli ;
Quesada, Pompeyo ;
Sahin, Ismet ;
Chandra, Vidhi ;
San Lucas, Anthony ;
Scheet, Paul ;
Xu, Hanwen ;
Hanash, Samir M. ;
Feng, Lei ;
Burks, Jared K. ;
Do, Kim-Anh ;
Peterson, Christine B. ;
Nejman, Deborah ;
Tzeng, Ching-Wei D. ;
Kim, Michael P. ;
Sears, Cynthia L. ;
Ajami, Nadim ;
Petrosino, Joseph ;
Wood, Laura D. ;
Maitra, Anirban ;
Straussman, Ravid ;
Katz, Matthew ;
White, James Robert ;
Jenq, Robert ;
Wargo, Jennifer ;
McAllister, Florencia .
CELL, 2019, 178 (04) :795-+
[28]   Circulating microbiome in blood of different circulatory compartments [J].
Schierwagen, Robert ;
Alvarez-Silva, Camila ;
Madsen, Mette Simone Aae ;
Kolbe, Carl Christian ;
Meyer, Carsten ;
Thomas, Daniel ;
Uschner, Frank Erhard ;
Magdaleno, Fernando ;
Jansen, Christian ;
Pohlmann, Alessandra ;
Praktiknjo, Michael ;
Hischebeth, Gunnar T. ;
Molitor, Ernst ;
Latz, Eicke ;
Lelouvier, Benjamin ;
Trebicka, Jonel ;
Arumugam, Manimozhiyan .
GUT, 2019, 68 (03) :578-580
[29]   Gut microbiome patterns correlate with higher postoperative complication rates after pancreatic surgery [J].
Schmitt, Felix C. F. ;
Brenner, Thorsten ;
Uhle, Florian ;
Loesch, Svenja ;
Hackert, Thilo ;
Ulrich, Alexis ;
Hofer, Stefan ;
Dalpke, Alexander H. ;
Weigand, Markus A. ;
Boutin, Sebastien .
BMC MICROBIOLOGY, 2019, 19 (1)
[30]   Metagenomic biomarker discovery and explanation [J].
Segata, Nicola ;
Izard, Jacques ;
Waldron, Levi ;
Gevers, Dirk ;
Miropolsky, Larisa ;
Garrett, Wendy S. ;
Huttenhower, Curtis .
GENOME BIOLOGY, 2011, 12 (06)