Microbiome Patterns in Matched Bile, Duodenal, Pancreatic Tumor Tissue, Drainage, and Stool Samples: Association with Preoperative Stenting and Postoperative Pancreatic Fistula Development

被引:19
作者
Langheinrich, Melanie [1 ]
Wirtz, Stefan [2 ]
Kneis, Barbara [1 ]
Gittler, Matthias M. [1 ]
Tyc, Olaf [3 ]
Schierwagen, Robert [3 ]
Brunner, Maximilian [1 ]
Krautz, Christian [1 ]
Weber, Georg F. [1 ]
Pilarsky, Christian [1 ]
Trebicka, Jonel [3 ]
Agaimy, Abbas [4 ]
Grutzmann, Robert [1 ]
Kersting, Stephan [1 ]
机构
[1] Univ Hosp Erlangen, Dept Surg, D-91054 Erlangen, Germany
[2] Univ Hosp Erlangen, Dept Internal Med 1, D-91054 Erlangen, Germany
[3] Univ Clin Frankfurt, Dept Internal Med 1, Translat Hepatol, D-60590 Frankfurt, Germany
[4] Univ Hosp Erlangen, Dept Pathol, D-91054 Erlangen, Germany
关键词
microbiome; pancreatic surgery; postoperative complications; pancreatic cancer; 16S rRNA sequencing; RISK SCORE; CANCER;
D O I
10.3390/jcm9092785
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Postoperative complications after pancreatic surgery are still a significant problem in clinical practice. The aim of this study was to characterize and compare the microbiomes of different body compartments (bile duct, duodenal mucosa, pancreatic tumor lesion, postoperative drainage fluid, and stool samples; preoperative and postoperative) in patients undergoing pancreatic surgery for suspected pancreatic cancer, and their association with relevant clinical factors (stent placement, pancreatic fistula, and gland texture). For this, solid (duodenal mucosa, pancreatic tumor tissue, stool) and liquid (bile, drainage fluid) biopsy samples of 10 patients were analyzed using 16s rRNA gene next-generation sequencing. Our analysis revealed: (i) a distinct microbiome in the different compartments, (ii) markedly higher abundance of Enterococcus in patients undergoing preoperative stent placement in the common bile duct, (iii) significant differences in the beta diversity between patients who developed a postoperative pancreatic fistula (POPF B/C), (iv) patients with POPF B/C were more likely to have bacteria belonging to the genus Enterococcus, and (v) differences in microbiome composition with regard to the pancreatic gland texture. The structure of the microbiome is distinctive in different compartments, and can be associated with the development of a postoperative pancreatic fistula.
引用
收藏
页码:1 / 17
页数:17
相关论文
共 38 条
[11]  
Fitzmaurice C., 2016, JAMA ONCOLOGY, V36, P1568, DOI DOI 10.1200/jco.2018.36.15_suppl.1568
[12]   The Influence of the Gut Microbiome on Cancer, Immunity, and Cancer Immunotherapy [J].
Gopalakrishnan, Vancheswaran ;
Helmink, Beth A. ;
Spencer, Christine N. ;
Reuben, Alexandre ;
Wargo, Jennifer A. .
CANCER CELL, 2018, 33 (04) :570-580
[13]   Fecal microbiome signatures of pancreatic cancer patients [J].
Half, Elizabeth ;
Keren, Nirit ;
Reshef, Leah ;
Dorfman, Tatiana ;
Lachter, Ishai ;
Kluger, Yoram ;
Reshef, Naama ;
Knobler, Hilla ;
Maor, Yaakov ;
Stein, Assaf ;
Konikoff, Fred M. ;
Gophna, Uri .
SCIENTIFIC REPORTS, 2019, 9 (1)
[14]   Oncobiosis and Microbial Metabolite Signaling in Pancreatic Adenocarcinoma [J].
Kiss, Borbala ;
Miko, Edit ;
Sebo, Eva ;
Toth, Judit ;
Ujlaki, Gyula ;
Szabo, Judit ;
Uray, Karen ;
Bai, Peter ;
Arkosy, Peter .
CANCERS, 2020, 12 (05)
[15]   The impact of surgical experience and frequency of practice on perioperative outcomes in pancreatic surgery [J].
Krautz, Christian ;
Haase, Elisabeth ;
Elshafei, Moustafa ;
Saeger, Hans-Detlev ;
Distler, Marius ;
Gruetzmann, Robert ;
Weber, Georg F. .
BMC SURGERY, 2019, 19 (01)
[16]   Bacterobilia in pancreatic surgery-conclusions for perioperative antibiotic prophylaxis [J].
Krueger, Colin Markus ;
Adam, Ulrich ;
Adam, Thomas ;
Kramer, Axel ;
Heidecke, Claus D. ;
Makowiec, Frank ;
Riediger, Hartwig .
WORLD JOURNAL OF GASTROENTEROLOGY, 2019, 25 (41) :6238-6247
[17]   The potential role of bacteria in pancreatic cancer: a systematic review [J].
Li, Ping ;
Shu, Yongqian ;
Gu, Yanhong .
CARCINOGENESIS, 2020, 41 (04) :397-404
[18]   Correlation between invasive microbiota in margin-surrounding mucosa and anastomotic healing in patients with colorectal cancer [J].
Li, Yan-Dong ;
He, Kang-Xin ;
Zhu, Wei-Fang .
WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY, 2019, 11 (09) :717-728
[19]   Alterations of the bile microbiome in primary sclerosing cholangitis [J].
Liwinski, Timur ;
Zenouzi, Roman ;
John, Clara ;
Ehlken, Hanno ;
Ruehlemann, Malte C. ;
Bang, Corinna ;
Groth, Stefan ;
Lieb, Wolfgang ;
Kantowski, Marcus ;
Andersen, Nils ;
Schachschal, Guido ;
Karlsen, Tom H. ;
Hov, Johannes R. ;
Roesch, Thomas ;
Lohse, Ansgar W. ;
Heeren, Joerg ;
Franke, Andre ;
Schramm, Christoph .
GUT, 2020, 69 (04) :665-672
[20]  
Martin M., 2011, EMBNET J, V17, P10, DOI [DOI 10.14806/EJ.17.1.200, 10.14806/ej.17.1.200]