Microbiome Patterns in Matched Bile, Duodenal, Pancreatic Tumor Tissue, Drainage, and Stool Samples: Association with Preoperative Stenting and Postoperative Pancreatic Fistula Development

被引:19
作者
Langheinrich, Melanie [1 ]
Wirtz, Stefan [2 ]
Kneis, Barbara [1 ]
Gittler, Matthias M. [1 ]
Tyc, Olaf [3 ]
Schierwagen, Robert [3 ]
Brunner, Maximilian [1 ]
Krautz, Christian [1 ]
Weber, Georg F. [1 ]
Pilarsky, Christian [1 ]
Trebicka, Jonel [3 ]
Agaimy, Abbas [4 ]
Grutzmann, Robert [1 ]
Kersting, Stephan [1 ]
机构
[1] Univ Hosp Erlangen, Dept Surg, D-91054 Erlangen, Germany
[2] Univ Hosp Erlangen, Dept Internal Med 1, D-91054 Erlangen, Germany
[3] Univ Clin Frankfurt, Dept Internal Med 1, Translat Hepatol, D-60590 Frankfurt, Germany
[4] Univ Hosp Erlangen, Dept Pathol, D-91054 Erlangen, Germany
关键词
microbiome; pancreatic surgery; postoperative complications; pancreatic cancer; 16S rRNA sequencing; RISK SCORE; CANCER;
D O I
10.3390/jcm9092785
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Postoperative complications after pancreatic surgery are still a significant problem in clinical practice. The aim of this study was to characterize and compare the microbiomes of different body compartments (bile duct, duodenal mucosa, pancreatic tumor lesion, postoperative drainage fluid, and stool samples; preoperative and postoperative) in patients undergoing pancreatic surgery for suspected pancreatic cancer, and their association with relevant clinical factors (stent placement, pancreatic fistula, and gland texture). For this, solid (duodenal mucosa, pancreatic tumor tissue, stool) and liquid (bile, drainage fluid) biopsy samples of 10 patients were analyzed using 16s rRNA gene next-generation sequencing. Our analysis revealed: (i) a distinct microbiome in the different compartments, (ii) markedly higher abundance of Enterococcus in patients undergoing preoperative stent placement in the common bile duct, (iii) significant differences in the beta diversity between patients who developed a postoperative pancreatic fistula (POPF B/C), (iv) patients with POPF B/C were more likely to have bacteria belonging to the genus Enterococcus, and (v) differences in microbiome composition with regard to the pancreatic gland texture. The structure of the microbiome is distinctive in different compartments, and can be associated with the development of a postoperative pancreatic fistula.
引用
收藏
页码:1 / 17
页数:17
相关论文
共 38 条
[1]   Preoperative Biliary Drainage of Severely Jaundiced Patients Increases Morbidity of Pancreaticoduodenectomy: Results of a Case-Control Study [J].
Arkadopoulos, Nikolaos ;
Kyriazi, Maria A. ;
Papanikolaou, Ioannis S. ;
Vasiliou, Pantelis ;
Theodoraki, Kassiani ;
Lappas, Christos ;
Oikonomopoulos, Nikolaos ;
Smyrniotis, Vassilios .
WORLD JOURNAL OF SURGERY, 2014, 38 (11) :2967-2972
[2]   A Prospectively Validated Clinical Risk Score Accurately Predicts Pancreatic Fistula after Pancreatoduodenectomy [J].
Callery, Mark P. ;
Pratt, Wande B. ;
Kent, Tara S. ;
Chaikof, Elliot L. ;
Vollmer, Charles M., Jr. .
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS, 2013, 216 (01) :1-14
[3]   Using MicrobiomeAnalyst for comprehensive statistical, functional, and meta-analysis of microbiome data [J].
Chong, Jasmine ;
Liu, Peng ;
Zhou, Guangyan ;
Xia, Jianguo .
NATURE PROTOCOLS, 2020, 15 (03) :799-821
[4]   MicrobiomeAnalyst: a web-based tool for comprehensive statistical, visual and meta-analysis of microbiome data [J].
Dhariwal, Achal ;
Chong, Jasmine ;
Habib, Salam ;
King, Irah L. ;
Agellon, Luis B. ;
Xia, Jianguo .
NUCLEIC ACIDS RESEARCH, 2017, 45 (W1) :W180-W188
[5]   Cancer of the pancreas: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-upaEuro [J].
Ducreux, M. ;
Cuhna, A. Sa. ;
Caramella, C. ;
Hollebecque, A. ;
Burtin, P. ;
Goere, D. ;
Seufferlein, T. ;
Haustermans, K. ;
Van Laethem, J. L. ;
Conroy, T. ;
Arnold, D. .
ANNALS OF ONCOLOGY, 2015, 26 :V56-V68
[6]  
Edgar R. C., 2016, bioRxiv, DOI 10.1101/074161
[7]  
Edgar RC, 2013, NAT METHODS, V10, P996, DOI [10.1038/NMETH.2604, 10.1038/nmeth.2604]
[8]   Search and clustering orders of magnitude faster than BLAST [J].
Edgar, Robert C. .
BIOINFORMATICS, 2010, 26 (19) :2460-2461
[9]   The cancer microbiome [J].
Elinav, Eran ;
Garrett, Wendy S. ;
Trinchieri, Giorgio ;
Wargo, Jennifer .
NATURE REVIEWS CANCER, 2019, 19 (07) :371-376
[10]   An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform [J].
Fadrosh, Douglas W. ;
Ma, Bing ;
Gajer, Pawel ;
Sengamalay, Naomi ;
Ott, Sandra ;
Brotman, Rebecca M. ;
Ravel, Jacques .
MICROBIOME, 2014, 2