Control of hepatocyte adhesion and function on self-organized honeycomb-patterned polymer film

被引：62

作者：

Tanaka, Masaru ^{[1
]}

Nishikawa, Kazutaka

Okubo, Hisashi

Kamachi, Hirofumi

Kawai, Tomoaki

Matsushita, Michiaki

Todo, Satoru

Shimomura, Masatsugu

机构：

[1] Hokkaido Univ, CRIS, Kita Ku, Sapporo, Hokkaido 0010021, Japan

[2] Japan Sci & Technol Corp, CREST, Kawaguchi 3320012, Japan

[3] Hokkaido Univ, Grad Sch Sci, Sapporo, Hokkaido 0010021, Japan

[4] Hokkaido Univ, Grad Sch Med, Sapporo, Hokkaido 0608638, Japan

[5] Hokkaido Univ, Res Inst Elect Sci, Nanotechnol Res Ctr, Kita Ku, Sapporo, Hokkaido 0010021, Japan

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2006年 / 284卷

关键词：

cell adhesion; hepatocyte; self-organization; hepatic function; cell surface interaction;

D O I：

10.1016/j.colsurfa.2005.11.098

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Hepatocytes were cultured on a honeycomb-patterned polymer film (honeycomb film) formed by self-organization in order to investigate the influence of the honeycomb pattern on cell behavior. The changes in cell morphologies and actin filaments were observed by optical, fluorescence, and scanning electron microscopy. Hepatocytes were flattened, and the actin filaments appeared conspicuously in the spreading regions on a flat film. In contrast, the hepatocytes that were cultured on the honeycomb film were observed to form a spherical shape, and the actin filaments were localized inside the edge of the spheroid. The spheroids were observed within several hours after seeding on the honeycomb film; they were attached and the spheroid shape was maintained without any deformation. The spheroids expressed a higher level of liver specific function than the cell monolayers on the flat film. These results suggest that the honeycomb film is a suitable material for tissue engineering scaffolds and biomedical devices. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：464 / 469

页数：6

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