LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness

被引：48

作者：

Zhu, Yihao ^{[1
]}

Hu, Hanqing ^{[1
]}

Yuan, Ziming ^{[1
]}

Zhang, Qian ^{[1
]}

Xiong, Huan ^{[1
]}

Hu, Zhiqiao ^{[1
]}

Wu, Hongyu ^{[1
]}

Huang, Rui ^{[1
]}

Wang, Guiyu ^{[1
]}

Tang, Qingchao ^{[1
]}

机构：

[1] Harbin Med Univ, Dept Colorectal Surg, Affiliated Hosp 2, Harbin, Peoples R China

来源：

CELL DEATH & DISEASE | 2020年 / 11卷 / 11期

关键词：

CELLS;

D O I：

10.1038/s41419-020-03164-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Resistance of chemotherapy is one of causes of recurrence and poor prognosis in patients with colorectal cancer (CRC). The role of differentially expressed long non-coding RNA (lncRNA) in 5-fluorouracil (5-Fu) resistance has not been fully elucidated. Here we observed that lncRNA NEAT1 was associated with 5-Fu resistance in CRC. Our Functional studies showed that NEAT1 promoted 5-Fu resistance in colorectal cells. In addition, A-TAC sequencing and chromatin immunoprecipitation (ChIP) showed that NEAT1 affected chromatin remodeling, increased the acetylation levels of histones, increased their enrichment at the promoters of ALDH1 and c-Myc, and promoted the expression of ALDH1 and c-Myc. Taken together, our study suggested that NEAT1 promoted 5-Fu resistance and cancer stemness by remodeling chromatin. Our finding provides a novel role of NEAT1 and may provide a new strategy for the treatment of CRC 5-Fu resistance.

引用

页数：11

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