Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder

被引：6
作者：
Liu, Yi [1 ,2 ]
Liu, Yongxin [3 ]
Liu, Yingjie [3 ]
Zhang, Xiaoyan [1 ]
Si, Fei [1 ]
Sun, Zhaohui [1 ]
Wang, Guixing [1 ]
Wang, Yufen [1 ]
Yang, Runqing [4 ]
Liu, Haijin [4 ]
机构：
[1] Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
[2] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China
[3] Chinese Acad Fishery Sci, Res Ctr Biotechnol, Beijing 100141, Peoples R China
[4] Chinese Acad Fishery Sci, Res Ctr Fisheries Recourse & Environm, Beijing 100141, Peoples R China
来源：
BIOLOGIA | 2013年 / 68卷 / 06期
关键词：
doubled haploid; linkage map; type II SSR; EST-SSR; Japanese flounder; skeletal traits; PARALICHTHYS-OLIVACEUS; SEQUENCES; PLANTS; CROSS; QTLS; DNA; L;
D O I：
10.2478/s11756-013-0265-4
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A genetic linkage map of Japanese flounder was constructed using 165 doubled haploids (DHs) derived from a single female. A total of 574 genomic microsatellites (type II SSRs) and expressed sequence tag (EST)-derived markers (EST-SSRs) were mapped to 24 linkage groups. The length of linkage map was estimated as 1270.9 centiMorgans (cM), with an average distance between markers of 2.2 cM. The EST-SSRs were used together with type II SSR markers to construct the Japanese flounder genetic linkage map which will facilitate identify quantitative trait locus (QTL) controlling important economic traits in Japanese flounder. Thus, twelve skeletal traits at 2 years of age were measured for all DHs. Forty-one QTLs were detected on 14 linkage groups and totally account for a small proportion of phenotypic variation (4.5 to 17.3%). Most of QTLs detected distribute on linkage groups 5 (9 QTLs), 8 (9 QTLs), 9 (5 QTLs) and 20 (4 QTLs), in which, some QTLs perform the pleiotropy.
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收藏
页码：1221 / 1228
页数：8
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