Bionanotechnology based on silica nanoparticles

被引:387
作者
Tan, WH [1 ]
Wang, KM
He, XX
Zhao, XJ
Drake, T
Wang, L
Bagwe, RP
机构
[1] Univ Florida, Dept Chem, Ctr Res Bionano Interface, Gainesville, FL 32611 USA
[2] Univ Florida, McKnights Brain Inst, Gainesville, FL 32611 USA
[3] Hunan Univ, Coll Chem & Chem Engn, Inst Biol Technol, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China
关键词
bionanotechnology; silica nanoparticles; nanoparticles;
D O I
10.1002/med.20003
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
We have developed uniform core/shell nanoparticles, consisting of a silica layer coating and pigments or magnetite core, using a water-in-oil microemulsion method. The nanoparticles are highly luminescent and photostable with the size ranging from 5 nm to 400 urn. Bioconjugation of these silica nanoparticles adds unique biofunctions with various molecules such as enzymes, antibodies, and DNA molecules. Significant advantages have been shown in using bioconjugated nanoparticles for biosensing and bioimaging, such as cell staining, DNA detection and separation, rapid single bacterium detection, and biotechnological application in DNA protection. (C) 2004 Wiley Periodicals, Inc.
引用
收藏
页码:621 / 638
页数:18
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