Field assessment of outcrossing from transgenic pea (Pisum sativum L.) plants

被引：12
|
作者：
Polowick, PL
Vandenberg, A
Mahon, JD
机构：
[1] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
[2] Univ Saskatchewan, Dept Agr, Ctr Crop Dev, Saskatoon, SK S7N 5A8, Canada
来源：
TRANSGENIC RESEARCH | 2002年 / 11卷 / 05期
关键词：
5-bromo-4-chloro-3-indolyl-beta-D-glucuronide; X-Gluc;
D O I：
10.1023/A:1020368322335
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The frequency of outcrossing from a transgenic line of peas into three cultivars ('Carneval', 'Montana', 'Tipu') was studied in the field in 1997 and 1999. Two dominant traits, normal leaf form and a highly-expressed glucuronidase (gusA) gene, were used as markers of pollen transfer. Because of heterogeneity in the commercial seed sources, leaf form alone was unreliable for assessing pollen migration into 'trap' plants. Of approximately 9000 offspring tested, only five plants scored positive for the presence of both markers. All five were located in 'trap' plots situated near the transgenic line. This represents a mean outcrossing rate of 0.07%.
引用
收藏
页码：515 / 519
页数：5
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