Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects

被引：26
作者：
Bastard, JP
Vidal, H
Jardel, C
Bruckert, E
Robin, D
Vallier, P
Blondy, P
Turpin, G
Forest, C
Hainque, B
机构：
[1] Hop La Pitie Salpetriere, Serv Biochim Pf J Delattre, AP HP, F-75013 Paris, France
[2] RTH Laennec, INSERM U449, Fac Med, Lyon, France
[3] Hop Pitie, Serv Endocrinol, AP HP, F-75651 Paris, France
[4] CNRS, Ctr Rech Endocrinol Mol & Dev, Meudon, France
[5] Grp Hosp Pitie Salpetriere, Inst Federatif Rech Coeur Muscle & Vaisseaux, AP HP, F-75634 Paris, France
来源：
INTERNATIONAL JOURNAL OF OBESITY | 2000年 / 24卷 / 01期
关键词：
adipocyte; obesity; plasminogen activator inhibitor-1; calorie restriction;
D O I：
10.1038/sj.ijo.0801087
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
OBJECTlVE: To determine whether changes in subcutaneous adipose tissue plasminogen activator inhibitor-1 (PAI-1) expression influence plasma PAI-7 level during weight: loss in obese humans. DESIGN: Study of the variations of PAI-1 levels both in plasma and in subcutaneous abdominal adipose tissue in 15 volunteer non-diabetic obese subjects, body mass index (BMI) 40.4. +/- 1.9 kg/m(2), aged 48 +/- 3 y, before and after a 3 week. very low calorie diet: (VLCD) programme (3.9+/- 0.1 MJ/day). MEASUREMENTS: Plasma and adipose tissue PAI-1 protein revels were measured by enzyme-linked immunosorbent assay and PAI-1 mRNA levels were quantified by quantitative RT-competitive PCR. RESULTS: VLCD induced weight loss (5.8+/-0.8kg) and decreased plasma)PAI-1 concentration (- 26% (P<0.01). Surprisingly, PAI-1 mRNA and protein abundance in subcutaneous adipose tissue increased by 87% (P < 0.05) and by 44% (P < 0.01). respectively. CONCLUSION: These data indicate thus that changes in subcutaneous adipose tissue PAI-1 expression are not involved in the decrease of plasma PAI-1 levels during VLCD in obese subjects.
引用
收藏
页码：70 / 74
页数：5
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