Lotus (Nelumbo nucifera) seed protein isolate exerts anti-inflammatory and antioxidant effects in LPS-stimulated RAW264.7 macrophages via inhibiting NF-κB and MAPK pathways, and upregulating catalase activity

被引：51
作者：
Moon, Seong Won [1 ]
Ahn, Chang-Bum [2 ]
Oh, Yunok [1 ]
Je, Jae-Young [1 ]
机构：
[1] Pukyong Natl Univ, Dept Marine Bio Convergence Sci, Busan 608739, South Korea
[2] Chonnam Natl Univ, Div Food & Nutr, Gwangju 550757, South Korea
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2019年 / 134卷
基金：
新加坡国家研究基金会;
关键词：
Nelumbo nucifera; Protein; Anti-inflammation; NF-kappa B; MAPK; RAW264.7; macrophages; MOLECULAR-BASIS; MYTILUS-EDULIS; MECHANISMS; FRACTION; CELLS; INFLAMMATION; EXTRACTS; NEFERINE; DISEASE; EMBRYO;
D O I：
10.1016/j.ijbiomac.2019.05.094
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lotus seed has long been used in traditional medicine and cuisine. In this study, lotus seed protein (LSP) was isolated and evaluated its anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. LSP isolate (LSPI) treatment in LPS-stimulated RAW264.7 macrophages resulted in the significant (p < 0.05) decrease of NO production by downregulation of the expressions of mRNA and protein, including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, LSPI treatment attenuated the production of reactive oxygen species (ROS) through increasing catalase activity, tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-1 beta in LPS-stimulated RAW264.7 macrophages. Furthermore, LPS stimulation in RAW264.7 macrophages caused the translocation of nuclear factor-kappa B (NF-kappa B) into the nucleus and the phosphorylation of mitogen-activated protein kinase (MAPK) but these stimulations were abolished by LSPI treatment. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码：791 / 797
页数：7
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