Comparison of the Bruker MALDI-TOF Mass Spectrometry System and Conventional Phenotypic Methods for Identification of Gram-Positive Rods

被引:64
作者
Barberis, Claudia [1 ]
Almuzara, Marisa [1 ]
Join-Lambert, Olivier [2 ,3 ]
Soledad Ramirez, Maria [4 ]
Famiglietti, Angela [1 ]
Vay, Carlos [1 ]
机构
[1] Univ Buenos Aires, Fac Farm & Bioquim, Hosp Clin Jose de San Martin, Inst Fisiopatol & Bioquim Clin, RA-1113 Buenos Aires, DF, Argentina
[2] Univ Paris 05, Paris, France
[3] Hop Necker Enfants Malad, Microbiol Lab, Paris, France
[4] Univ Buenos Aires, Consejo Nacl Invest Cient & Tecn, Inst Microbiol & Parasitol Med, Buenos Aires, DF, Argentina
关键词
DESORPTION IONIZATION-TIME; CORYNEFORM BACTERIA; CORYNEBACTERIUM; RPOB;
D O I
10.1371/journal.pone.0106303
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In recent years, MALDI-TOF Mass Spectrometry (MS) method has emerged as a promising and a reliable tool for bacteria identification. In this study we compared Bruker MALDI-TOF MS and conventional phenotypic methods to identify a collection of 333 Gram-positive clinical isolates comprising 22 genera and 60 species. 16S rRNA sequencing was the reference molecular technique, and rpoB gene sequecing was used as a secondary gene target when 16Sr RNA did not allow species identification of Corynebacterium spp. We also investigate if score cut-offs values of >= 1,5 and >= 1,7 were accurate for genus and species-level identification using the Bruker system. Identification at species level was obtained for 92,49% of Gram-positive rods by MALDI-TOF MS compared to 85,89% by phenotypic method. Our data validates the score >= 1,5 for genus level and >= 1,7 for species-level identification in a large and diverse collection of Gram-positive rods. The present study has proved the accuracy of MALDI-TOF MS as an identification method in Gram-positive rods compared to currently used methods in routine laboratories.
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