Adhesiveness of opportunistic Staphylococcus aureus to materials used in dental office: In vitro study

被引：7

作者：

Merghni, Abderrahmen ^{[1
]}

Bekir, Karima ^{[2
]}

Kadmi, Yassine ^{[3
,4
,5
,6
]}

Dallel, Ines ^{[7
]}

Janel, Sebastien ^{[8
]}

Bovio, Simone ^{[8
]}

Barois, Nicolas ^{[8
]}

Lafont, Frank ^{[8
,9
]}

Mastouri, Maha ^{[1
,10
]}

机构：

[1] Monastir Univ, Fac Pharm, Lab Infect Dis & Biol Agents LR99ES27, Monastir, Tunisia

[2] Monastir Univ, Fac Pharm, Lab Anal Treatment & Valorizat Environm Pollutant, Monastir, Tunisia

[3] Univ Artois, EA 7394, Inst Charles VIOLLETTE, F-62300 Lens, France

[4] ISA Lille, EA 7394, Inst Charles VIOLLETTE, F-59000 Lille, France

[5] Ulco, EA 7394, Inst Charles VIOLLETTE, F-62200 Boulogne Sur Mer, France

[6] Univ Lille, EA 7394, Inst Charles VIOLLETTE, F-59000 Lille, France

[7] Monastir Dent Clin, Dept Orthodont, Lab Oral Hlth & Orofacial Rehabil LR12ES11, Monastir, Tunisia

[8] BioImaging Ctr Lille, FR3642, Lille, France

[9] Univ Lille, Lille Reg Univ Hosp Ctr, Inst Pasteur Lille,Cellular Microbiol & Phys Infe, INSERM U1019,CNRS UMR8204,Ctr Infect & Immun Lill, Lille, France

[10] Univ Hosp Fattouma Bourguiba, Microbiol Lab, Monastir, Tunisia

来源：

MICROBIAL PATHOGENESIS | 2017年 / 103卷

关键词：

Inert materials; Surface characteristics; Adhesion forces; Biofilm; Staphylococcus aureus; BACTERIAL ADHESION FORCES; SURFACE FREE-ENERGY; STAINLESS-STEEL; BIOFILM FORMATION; CANDIDA-ALBICANS; CONTAMINATION; QUANTIFICATION; TOPOGRAPHY; ROUGHNESS; FOOD;

D O I：

10.1016/j.micpath.2016.12.014

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Staphylococcus aureus (S. aureus) is one of several opportunistic microbial pathogens associated with many healthcare problems. In the present study, S. aureus was assessed for its biofilm-forming ability on materials routinely used in dental offices, including stainless steel (SS), polyethylene (PE), and polyvinyl chloride (PVC). Materials that were tested were characterized for roughness (Ra) and surface free energy (SFE). The adhesion forces exerted by S. aureus to each substratum were investigated using atomic force microscopy (AFM), and biofilm formation was quantitatively assessed by crystal violet staining assay. AFM measurements demonstrated that the strongest adhesion forces (20 nN) were exerted on the PE surfaces (P < 0.05) and depended more on Ra. In addition, the results of biofilm formation capability indicated that S. aureus exhibited more affinity to SS materials when compared to the other materials (P < 0.05). This ability of biofilm formation seems to be more correlated to SFE (R = 0.65). Hence, control of the surface properties of materials used in dental practices is of crucial importance for preventing biofilm formation on dental materials to be used for patients' dental care. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：129 / 134

页数：6

共 41 条

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