The endocytic protein α-adaptin is required for numb-mediated asymmetric cell division in Drosophila

被引：298
作者：
Berdnik, D
Török, T
González-Gaitán, M
Knoblich, JA
机构：
[1] Res Inst Mol Pathol, IMP, A-1030 Vienna, Austria
[2] Max Planck Inst Mol Zellbiol & Genet, D-01307 Dresden, Germany
来源：
DEVELOPMENTAL CELL | 2002年 / 3卷 / 02期
关键词：
D O I：
10.1016/S1534-5807(02)00215-0
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
During asymmetric cell division in Drosophila sensory organ precursor cells, the Numb protein localizes asymmetrically and segregates into one daughter cell, where it influences cell fate by repressing signal transduction via the Notch receptor. We show here that Numb acts by polarizing the distribution of alpha-Adaptin, a protein involved in receptor-mediated endocytosis. a-Adaptin binds to Numb and localizes asymmetrically in a Numb-dependent fashion. Mutant forms of a-Adaptin that no longer bind to Numb fail to localize asymmetrically and cause numb-like defects in asymmetric cell division. Our results suggest a model in which Numb influences cell fate by downregulating Notch through polarized receptor-mediated endocytosis, since Numb also binds to the intracellular domain of Notch.
引用
收藏
页码：221 / 231
页数：11
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