Gene expression of Interleukin-18 in rheumatoid arthritis patients on disease modifying anti-rheumatic drug therapy

被引：4

作者：

Khalid, Sabeen ^{[1
]}

Yousaf, Muhammad Javad ^{[2
]}

Rashid, Amir ^{[2
]}

Khan, Saleem Ahmad ^{[3
]}

机构：

[1] Aziz Fatimah Med & Dent Coll, Dept Biochem, Faisalabad, Pakistan

[2] Army Med Coll, Dept Biochem & Mol Biol, Rawalpindi, Pakistan

[3] Army Med Coll, Dept Pathol, Rawalpindi, Pakistan

来源：

PAKISTAN JOURNAL OF MEDICAL SCIENCES | 2019年 / 35卷 / 03期

关键词：

Rheumatoid arthritis; Interleukin-18; Disease modifying anti-rheumatic drugs; Gene expression;

D O I：

10.12669/pjms.35.3.1070

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background & Objectives: The hallmark of rheumatoid arthritis is the inflammation that is mediated by the macrophages and monocytes that cause release of pro-inflammatory cytokines like interleukin-18. It is highly expressed in serum of patients suffering from rheumatoid arthritis and has a positive association with disease activity. The aim of this study was to analyze the gene expression of interleukin-18 in rheumatoid arthritis patients on disease modifying anti-rheumatic drug therapy. Methods: The cross sectional comparative study is conducted at Department of Biochemistry and Molecular Biology and Center for Research in Experimental and Applied Medicine (CREAM-1Lab), Army Medical College, Rawalpindi, in collaboration with Rheumatology Department, Military Hospital, Rawalpindi. Study was conducted on two groups consisting of Group-I of rheumatoid arthritis patients on diseases modifying antirheumatic drugs and control Group-II comprising of normal healthy individuals. Non-probability purposive sampling was done from patients and controls. The duration of study was one year i-e from November 2015 to November 2016. Relative quantification of gene expression of interleukin-18 was done by Real time PCR using Delta Delta CT method. Results: Expression analysis for interleukin-18 showed down regulation of gene in rheumatoid arthritis patients as compared to controls. Conclusion: Interleukin-18 gene shows down regulation in rheumatoid arthritis patients on disease modifying anti-rheumatic drugs therapy.

引用

页码：802 / 806

页数：5

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