Cell-specific gene expression of phosphate transporters in mycorrhizal tomato roots

被引:37
作者
Gomez-Ariza, Jorge [1 ,2 ]
Balestrini, Raffaella [1 ,2 ]
Novero, Mara [1 ,2 ]
Bonfante, Paola [1 ,2 ]
机构
[1] Univ Turin, Dept Plant Biol, I-10125 Turin, Italy
[2] CNR, Inst Plant Protect, I-10125 Turin, Italy
关键词
Arbuscular mycorrhizae; Phosphate transporters; Laser microdissection; Cell specificity; Tomato; Glomus mosseae; MEDICAGO-TRUNCATULA; LASER MICRODISSECTION; CORTICAL-CELLS; PLANT; LYCOPERSICON; APPARATUS; SYMBIOSIS; FUNGI;
D O I
10.1007/s00374-009-0399-2
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Phosphorus (P) is an essential plant nutrient and is limiting for plant growth in most natural ecosystems as well as in agricultural production throughout the world. In arbuscular mycorrhizal (AM) symbiosis, the fungus obtains carbon from the plant and transfers mineral nutrients, like phosphate (Pi), from the soil to the root cells. Mycorrhizal plants can acquire Pi directly from the soil through plant-specific Pi transporters (the direct uptake pathway) or through fungal uptake and transport systems (the mycorrhizal uptake pathway). The study of plant Pi transporters and of their functioning therefore represents a key point for plant physiology. In this work, after a morphological analysis of tomato mycorrhizal roots, we have used the laser microdissection technology to collect cortical, epidermal, and central cylinder cells with the aim of analyzing the expression of the five so far identified tomato Pi transporter genes in the different root tissues. Using reverse transcriptase PCR analysis, LePT1 and LePT2 transcripts were detected in the epidermal and cortical cells, while LePT3, LePT4, and LePT5 transcripts were confirmed to be exclusive of arbuscule-containing cells. Despite the presence of mRNA in all the analyzed cell populations, no expression of the known tomato Pi transporter genes was observed in the central cylinder cells, irrespective of the presence of the AM fungus.
引用
收藏
页码:845 / 853
页数:9
相关论文
共 38 条
[1]   Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells [J].
Balestrini, Raffaella ;
Gomez-Ariza, Jorge ;
Lanfranco, Luisa ;
Bonfante, Paola .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2007, 20 (09) :1055-1062
[2]   Application of Laser Microdissection to plant pathogenic and symbiotic interactions [J].
Balestrini, Raffaella ;
Gomez-Ariza, Jorge ;
Klink, Vincent P. ;
Bonfante, Paola .
JOURNAL OF PLANT INTERACTIONS, 2009, 4 (02) :81-92
[3]   Expression profiles of a phosphate transporter gene (GmosPT) from the endomycorrhizal fungus Glomus mosseae [J].
Benedetto, A ;
Magurno, F ;
Bonfante, P ;
Lanfranco, L .
MYCORRHIZA, 2005, 15 (08) :620-627
[4]  
BONFANTE P, 2009, MYCOTA, V5, P257
[5]   NUCLEOTIDE-SEQUENCES OF 2 PEROXIDASE GENES FROM TOMATO (LYCOPERSICON-ESCULENTUM) [J].
BOTELLA, MA ;
QUESADA, MA ;
HASEGAWA, PM ;
VALPUESTA, V .
PLANT PHYSIOLOGY, 1993, 103 (02) :665-666
[6]   The expression of an extensin-like protein correlates with cellular tip growth in tomato [J].
Bucher, M ;
Brunner, S ;
Zimmermann, P ;
Zardi, GI ;
Amrhein, N ;
Willmitzer, L ;
Riesmeier, JW .
PLANT PHYSIOLOGY, 2002, 128 (03) :911-923
[7]   Functional biology of plant phosphate uptake at root and mycorrhiza interfaces [J].
Bucher, Marcel .
NEW PHYTOLOGIST, 2007, 173 (01) :11-26
[8]   The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface [J].
Chiou, TJ ;
Liu, H ;
Harrison, MJ .
PLANT JOURNAL, 2001, 25 (03) :281-293
[9]   Functional analysis and cell-specific expression of a phosphate transporter from tomato [J].
Daram, P ;
Brunner, S ;
Persson, BL ;
Amrhein, N ;
Bucher, M .
PLANTA, 1998, 206 (02) :225-233
[10]   Laser capture microdissection [J].
EmmertBuck, MR ;
Bonner, RF ;
Smith, PD ;
Chuaqui, RF ;
Zhuang, ZP ;
Goldstein, SR ;
Weiss, RA ;
Liotta, LA .
SCIENCE, 1996, 274 (5289) :998-1001