Photon statistics and dynamics of fluorescence resonance energy transfer

被引：70
作者：
Berglund, AJ [1 ]
Doherty, AC [1 ]
Mabuchi, H [1 ]
机构：
[1] CALTECH, Norman Bridge Lab Phys 12 33, Pasadena, CA 91125 USA
来源：
PHYSICAL REVIEW LETTERS | 2002年 / 89卷 / 06期
关键词：
D O I：
10.1103/PhysRevLett.89.068101
中图分类号：
O4 [物理学];
学科分类号：
0702 ;
摘要：
We report high time-resolution measurements of photon statistics from pairs of dye molecules coupled by fluorescence resonance energy transfer (FRET). In addition to quantum-optical photon antibunching, we observe photon bunching on a time scale of several nanoseconds. We show by numerical simulation that configuration fluctuations in the coupled fluorophore system could account for minor deviations of our data from predictions of basic Forster theory. With further characterization we believe that FRET photon statistics could provide a unique tool for studying DNA mechanics on time scales from 10(-9)-10(-3) s.
引用
收藏
页码：1 / 068101
页数：4
相关论文
共 50 条
  • [21] NONCLASSICAL PHOTON STATISTICS IN SMOOTH-PULSE RESONANCE FLUORESCENCE
    FLORJANCZYK, M
    [J]. JOURNAL OF PHYSICS B-ATOMIC MOLECULAR AND OPTICAL PHYSICS, 1987, 20 (01) : 37 - 41
  • [22] PHOTON-COUNTING STATISTICS OF RESONANCE FLUORESCENCE IN A SQUEEZED VACUUM
    JAGATAP, BN
    LAWANDE, SV
    [J]. PHYSICAL REVIEW A, 1991, 44 (09): : 6030 - 6035
  • [23] Subpopulation-resolved photon statistics of single-molecule energy transfer dynamics
    Nettels, Daniel
    Schuler, Benjamin
    [J]. IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 2007, 13 (04) : 990 - 995
  • [24] Comparison of one- and two-photon fluorescence resonance energy transfer microscopy
    Periasamy, A
    Elangovan, M
    Day, RN
    [J]. MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES, 2001, 4262 : 366 - 372
  • [25] Homogeneous immunoassay based on two-photon excitation fluorescence resonance energy transfer
    Liu, Lingzhi
    Shao, Mei
    Dong, Xiaohu
    Yu, Xuefeng
    Liu, Zhihong
    He, Zhike
    Wang, Ququan
    [J]. ANALYTICAL CHEMISTRY, 2008, 80 (20) : 7735 - 7741
  • [26] Measuring distances within unfolded biopolymers using fluorescence resonance energy transfer: The effect of polymer chain dynamics on the observed fluorescence resonance energy transfer efficiency
    Makarov, Dmitrii E.
    Plaxco, Kevin W.
    [J]. JOURNAL OF CHEMICAL PHYSICS, 2009, 131 (08)
  • [27] FLUORESCENCE RESONANCE ENERGY-TRANSFER
    SELVIN, PR
    [J]. BIOCHEMICAL SPECTROSCOPY, 1995, 246 : 300 - 334
  • [28] Fluorescence resonance energy transfer sensors
    Birch, DJS
    Rolinski, OJ
    [J]. RESEARCH ON CHEMICAL INTERMEDIATES, 2001, 27 (4-5) : 425 - 446
  • [29] Cytometry of fluorescence resonance energy transfer
    Vereb, G
    Matkó, J
    Szöllösi, J
    [J]. CYTOMETRY, 4TH EDITION: NEW DEVELOPMENTS, 2004, 75 : 105 - 152
  • [30] Fluorescence resonance energy transfer sensors
    D J S Birch
    O J Rolinski
    [J]. Research on Chemical Intermediates, 2001, 27 : 425 - 446
12345