CD44 AND CD24 CANNOT ACT AS CANCER STEM CELL MARKERS IN HUMAN LUNG ADENOCARCINOMA CELL LINE A549

被引:41
作者
Roudi, Raheleh [1 ,2 ,4 ]
Madjd, Zahra [1 ,3 ,4 ]
Ebrahimi, Marzieh [2 ]
Samani, Fazel Sahraneshin [2 ]
Samadikuchaksaraei, Ali [4 ,5 ]
机构
[1] Iran Univ Med Sci, Fac Adv Technol Med, Dept Mol Med, Tehran, Iran
[2] ACECR, Royan Inst Stem Cell Biol & Technol, Dept Stem Cells & Dev Biol, Cell Sci Res Ctr, Tehran, Iran
[3] Iran Univ Med Sci, Oncopathol Res Ctr, Tehran, Iran
[4] Iran Univ Med Sci, Cellular & Mol Res Ctr, Tehran, Iran
[5] Iran Univ Med Sci, Dept Med Biotechnol, Tehran, Iran
关键词
Cancer stem cells; Lung cancer; Cell line A549; Colony-formation assay; Sphere-formation assay; CD44; CD24; CD133; ALDH1; ABCG-2; IDENTIFICATION; SUBPOPULATIONS; POPULATION; CD133(+); DISPLAY; TUMORS; ASSAY;
D O I
10.2478/s11658-013-0112-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cancer stem cells (CSCs) are subpopulations of tumor cells that are responsible for tumor initiation, maintenance and metastasis. Recent studies suggested that lung cancer arises from CSCs. In this study, the expression of potential CSC markers in cell line A549 was evaluated. We applied flow cytometry to assess the expression of putative stem cell markers, including aldehyde dehydrogenase 1 (ALDH1), CD24, CD44, CD133 and ABCG2. Cells were then sorted according to the expression of CD44 and CD24 markers by fluorescence-activated cell sorting (FACS) Aria II and characterized using their clonogenic and sphere-forming capacity. A549 cells expressed the CSC markers CD44 and CD24 at 68.16% and 54.46%, respectively. The expression of the putative CSC marker ALDH1 was 4.20%, whereas the expression of ABCG2 and CD133 was 0.93%. Double-positive CD44/133 populations were rare. CD44(+)/24(+) and CD44(+)/CD24(-/low) subpopulations respectively exhibited 64% and 27.92% expression. The colony-forming potentials in the CD44(+)/CD24(+) and CD44(+)/CD24(-/low) subpopulations were 84.37 +/- 2.86% and 90 +/- 3.06%, respectively, while the parental A549 cells yielded 56.65 +/- 2.33% using the colony-formation assay. Both isolated subpopulations formed spheres in serum-free medium supplemented with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). CD44 and CD24 cannot be considered potential markers for isolating lung CSCs in cell line A549, but further investigation using in vivo assays is required.
引用
收藏
页码:23 / 36
页数:14
相关论文
共 42 条
  • [1] Non-small cell lung cancer stem/progenitor cells are enriched in multiple distinct phenotypic subpopulations and exhibit plasticity
    Akunuru, S.
    Zhai, Q. James
    Zheng, Y.
    [J]. CELL DEATH & DISEASE, 2012, 3 : e352 - e352
  • [2] Prospective identification of tumorigenic breast cancer cells
    Al-Hajj, M
    Wicha, MS
    Benito-Hernandez, A
    Morrison, SJ
    Clarke, MF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) : 3983 - 3988
  • [3] 3 CLONAL TYPES OF KERATINOCYTE WITH DIFFERENT CAPACITIES FOR MULTIPLICATION
    BARRANDON, Y
    GREEN, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (08) : 2302 - 2306
  • [4] Highly tumorigenic lung cancer CD133+ cells display stem-like features and are spared by cisplatin treatment
    Bertolini, Giulia
    Roz, Luca
    Perego, Paola
    Tortoreto, Monica
    Fontanella, Enrico
    Gatti, Laura
    Pratesi, Graziella
    Fabbri, Alessandra
    Andriani, Francesca
    Tinelli, Stella
    Roz, Elena
    Caserini, Roberto
    Lo Vullo, Salvatore
    Camerini, Tiziana
    Mariani, Luigi
    Delia, Domenico
    Calabro, Elisa
    Pastorino, Ugo
    Sozzi, Gabriella
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (38) : 16281 - 16286
  • [5] Cancer stem cells: A step toward the cure
    Boman, Bruce M.
    Wicha, Max S.
    [J]. JOURNAL OF CLINICAL ONCOLOGY, 2008, 26 (17) : 2795 - 2799
  • [6] The increasing incidence of lung adenocarcinoma: Reality or artefact? A review of the epidemiology of lung adenocarcinoma
    Charloux, A
    Quoix, E
    Wolkove, N
    Small, D
    Pauli, G
    Kreisman, H
    [J]. INTERNATIONAL JOURNAL OF EPIDEMIOLOGY, 1997, 26 (01) : 14 - 23
  • [7] Oct-4 Expression Maintained Cancer Stem-Like Properties in Lung Cancer-Derived CD133-Positive Cells
    Chen, Yu-Chih
    Hsu, Han-Shui
    Chen, Yi-Wei
    Tsai, Tung-Hu
    How, Chorng-Kuang
    Wang, Chien-Ying
    Hung, Shih-Chieh
    Chang, Yuh-Lih
    Tsai, Ming-Long
    Lee, Yi-Yen
    Ku, Hung-Hai
    Chiou, Shih-Hwa
    [J]. PLOS ONE, 2008, 3 (07):
  • [8] ABCG2: A potential marker of stem cells and novel target in stern cell and cancer therapy
    Ding, Xi-wei
    Wu, Jun-hua
    Jiang, Chun-ping
    [J]. LIFE SCIENCES, 2010, 86 (17-18) : 631 - 637
  • [9] Identification and expansion of the tumorigenic lung cancer stem cell population
    Eramo, A.
    Lotti, F.
    Sette, G.
    Pilozzi, E.
    Biffoni, M.
    Di Virgilio, A.
    Conticello, C.
    Ruco, L.
    Peschle, C.
    De Maria, R.
    [J]. CELL DEATH AND DIFFERENTIATION, 2008, 15 (03) : 504 - 514
  • [10] Nomenclature of prominin-1 (CD133) splice variants - an update
    Fargeas, C. A.
    Huttner, W. B.
    Corbeil, D.
    [J]. TISSUE ANTIGENS, 2007, 69 (06): : 602 - 606