Immunoassays and sequence-specific DNA detection on a microchip using enzyme amplified electrochemical detection

被引：38

作者：

Dill, K ^{[1
]}

Montgomery, DD ^{[1
]}

Ghindilis, AL ^{[1
]}

Schwarzkopf, KR ^{[1
]}

机构：

[1] CombiMatrix Corp, Mukilteo, WA 98275 USA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2004年 / 59卷 / 02期

关键词：

microarray; multiplexed assays; protein and DNA detection; enzyme amplified electrochemical detection;

D O I：

10.1016/j.jbbm.2004.01.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A CMOS fabricated silicon microchip was used as a platform for immunoassays and DNA synthesis and hybridization. The chip is covered with a biofriendly matrix wherein the chemistries occur. The active silicon chip has over 1000 active electrodes that can be individually addressed for both synthesis of DNA and protein attachment to a membrane on the chip surface. Additionally, the active chip can be further used for the detection of various analytes at the chip surface via digital read out resulting from the redox enzymes on the captured oligonucleotide or antibody. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：181 / 187

页数：7

共 18 条

[1] Protein microchips: Use for immunoassay and enzymatic reactions [J].