Effect of Low-Level Laser on Bone Defects Treated with Bovine or Autogenous Bone Grafts: In Vivo Study in Rat Calvaria

被引：30

作者：

Cunha, Mercia J. S. ^{[1
,2
,3
]}

Esper, Luis A. ^{[2
,3
]}

Sbrana, Michyele C. ^{[2
,3
]}

de Oliveira, Paula G. F. P. ^{[1
,3
]}

do Valle, Accacio L. ^{[3
,4
]}

de Almeida, Ana Lucia P. F. ^{[3
,4
]}

机构：

[1] Univ Sao Paulo, Hosp Rehabil Craniofacial Anomalies, BR-17012901 Bauru, SP, Brazil

[2] Univ Sao Paulo, Bauru Sch Dent, Sao Paulo, Brazil

[3] Univ Sao Paulo, Fac Odontol Bauru, BR-17012901 Bauru, SP, Brazil

[4] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont, Sao Paulo, Brazil

来源：

BIOMED RESEARCH INTERNATIONAL | 2014年 / 2014卷

基金：

巴西圣保罗研究基金会;

关键词：

REPAIR PROCESS; GROWTH-FACTORS; THERAPY; IRRADIATION; PROLIFERATION; REGENERATION; SUBSTITUTES; DIFFERENTIATION; BIO-OSS(R); IMPLANTS;

D O I：

10.1155/2014/104230

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Objective. The purpose of this study was to histologically evaluate the effect of low-level laser (LLL) on the healing of critical size defects (CSD) in rat calvaria, filled with autogenous or inorganic bovine bone grafts. Methods. Sixty rats were divided into 6 groups (n = 10): C (control-filled with blood clot), LLL (low-level laser-GaAlAs, lambda 780 nm, 100 mW, 210 J/cm(2), Phi 0.05 cm(2); 6 J/point), AB (autogenous bone), ABL (autogenous bone + low-level laser), OB (inorganic bovine bone), and OBL (inorganic bovine bone + LLL). Material and Methods. The animals were killed after 30 days. Histological and histometric analyses were performed by light microscopy. Results. The groups irradiated with laser, LLL (47.67% +/- 8.66%), ABL (39.15% +/- 16.72%), and OBL (48.57% +/- 28.22%), presented greater area of new bone formation than groups C (9.96% +/- 4.50%), AB (30.98% +/- 16.59%), and OB (11.36% +/- 7.89%), which were not irradiated. Moreover, they were significantly better than group C (Kruskal-Wallis test followed by Dunn test, P < 0.05). Conclusion. The laser accelerated the healing of bone defects and the resorption of particles of the graft material.

引用

页数：9