A highly selective turn-off fluorescent probe for Cu(II) based on a dansyl derivative and its application in living cell imaging

被引：47

作者：

Huang, Jiaguo ^{[1
]}

Liu, Min ^{[1
]}

Ma, Xiaoqian ^{[2
]}

Dong, Qiong ^{[2
]}

Ye, Bin ^{[2
]}

Wang, Wei ^{[2
]}

Zeng, Wenbin ^{[1
]}

机构：

[1] Cent S Univ, Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China

[2] Cent S Univ, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China

来源：

RSC ADVANCES | 2014年 / 4卷 / 44期

基金：

中国国家自然科学基金;

关键词：

COPPER; SENSOR; DEPOSITION; CONJUGATE; ZINC; HG2+; BOG;

D O I：

10.1039/c4ra02050b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel dansyl derivative (1) was designed and synthesized with high yield. It is highly selective for Cu2+ over other competing metal ions such as Ca2+, Co2+, Cr3+, Cu+, Fe2+, Ga3+, Hg2+, Mg2+, Na+, Ni2+ and Pb2+. Zn2+ and Fe3+ only slightly changed the fluorescence of probe 1. The linear relationship between fluorescence intensity and Cu2+ concentration indicates that 1 can be used for quantification. The binding ratio of probe 1 to Cu2+ was found to be 1 : 1 according to Job's plot experiments. Probe 1 can be used in a broad pH value window ranging from 5 to 11. The limit of detection ( LOD) based on 3 x delta(blank)/k was calculated with a value as low as 1.6 x 10(-6) M for Cu2+. Additionally, the association constant of probe 1 - Cu2+ complexes was found to be 5.08 x 104 M-1. Moreover, fluorescence microscopy experiments showed that 1 can be used as a fluorescent probe for evaluating the presence of exogenous Cu2+ in living cells.

引用

页码：22964 / 22970

页数：7

共 31 条

[1] Neurodegenerative diseases and oxidative stress [J].

Barnham, KJ ;

Masters, CL ;

Bush, AI .

NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (03) :205-214

[2] The biogeochemistry of an ombrotrophic bog: Evaluation of use as an archive of atmospheric mercury deposition [J].

Benoit, JM ;

Fitzgerald, WF ;

Damman, AWH .

ENVIRONMENTAL RESEARCH, 1998, 78 (02) :118-133

[3] A two-channel molecular dosimeter for the optical detection of copper(II) [J].

Boiocchi, M ;

Fabbrizzi, L ;

Licchelli, M ;

Sacchi, D ;

Vázquez, M ;

Zampa, C .

CHEMICAL COMMUNICATIONS, 2003, (15) :1812-1813

[4] A Highly Selective Turn-On Colorimetric, Red Fluorescent Sensor for Detecting Mobile Zinc in Living Cells [J].

Du, Pingwu ;

Lippard, Stephen J. .

INORGANIC CHEMISTRY, 2010, 49 (23) :10753-10755

[5] Energy transfer cassettes based on organic fluorophores: construction and applications in ratiometric sensing [J].

Fan, Jiangli ;

Hu, Mingming ;

Zhan, Peng ;

Peng, Xiaojun .

CHEMICAL SOCIETY REVIEWS, 2013, 42 (01) :29-43

[6] A FRET-based rhodamine-benzimidazole conjugate as a Cu2+-selective colorimetric and ratiometric fluorescence probe that functions as a cytoplasm marker [J].

Goswami, Shyamaprosad ;

Maity, Sibaprasad ;

Maity, Annada C. ;

Maity, Anup Kumar ;

Das, Avijit Kumar ;

Saha, Partha .

RSC ADVANCES, 2014, 4 (12) :6300-6305

[7] Ratiometric fluorescence sensing and cellular imaging of Cu2+ by a new water soluble trehalose-naphthalimide based chemosensor [J].

Grasso, Giuseppa Ida ;

Gentile, Salvatore ;

Giuffrida, Maria Laura ;

Satriano, Cristina ;

Sgarlata, Carmelo ;

Sgarzi, Massimo ;

Tomaselli, Gaetano ;

Arena, Giuseppe ;

Prodi, Luca .

RSC ADVANCES, 2013, 3 (46) :24288-24297

[8] Hematological manifestations of copper deficiency: a retrospective review [J].

Halfdanarson, Thorvardur R. ;

Kumar, Neeraj ;

Li, Chin-Yang ;

Phyliky, Robert L. ;

Hogan, William J. .

EUROPEAN JOURNAL OF HAEMATOLOGY, 2008, 80 (06) :523-531

[9] A Rhodamine-Based Hg2+ Sensor with High Selectivity and Sensitivity in Aqueous Solution: A NS2-Containing Receptor [J].

Huang, Junhai ;

Xu, Yufang ;

Qian, Xuhong .

JOURNAL OF ORGANIC CHEMISTRY, 2009, 74 (05) :2167-2170

[10] Selective colorimetric sensing of Cu2+ using triazolyl monoazo derivative [J].

Kaur, Paramjit ;

Sareen, Divya ;

Singh, Kamaljit .

TALANTA, 2011, 83 (05) :1695-1700

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