Preparation and preliminary study of crystals of the recombinant calcium-regulated photoprotein obelin from the bioluminescent hydroid Obelia longissima

被引：8
作者：
Vysotski, ES
Liu, ZJ
Rose, J
Wang, BC
Lee, J [1 ]
机构：
[1] Russian Acad Sci, Photobiol Lab, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[2] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444999011828
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Crystals of recombinant obelin, the Ca2+-regulated photoprotein from the marine hydroid Obelia longissima, have been grown from sodium citrate solutions. Crystals grow as hexagonal light-yellow rods (0.1 x 0.1 x 1.0 mm) which diffract to beyond 1.8 Angstrom with synchrotron radiation of 1.0 Angstrom wavelength. The crystals have a primitive hexagonal lattice with unit-cell parameters a = 81.55, c = 86.95 Angstrom. The asymmetric unit contains two molecules. This represents the successful preparation of single crystals of a photoprotein obelin which have promising diffraction properties.
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页码：1965 / 1966
页数：2
相关论文
共 23 条
[1]  
[Anonymous], 1966, Bioluminescence in Progress
[2]  
Blinks J.R., 1986, SARCOPLASMIC RETICUL, VII, P73
[3]   MEASUREMENT OF CA-2+ CONCENTRATIONS IN LIVING CELLS [J].
BLINKS, JR ;
WIER, WG ;
HESS, P ;
PRENDERGAST, FG .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1982, 40 (1-2) :1-114
[4]  
BONDAR VS, 1992, BIOCHEMISTRY-MOSCOW+, V57, P1020
[5]   Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes [J].
Conti, E ;
Franks, NP ;
Brick, P .
STRUCTURE, 1996, 4 (03) :287-298
[6]   3-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO-HARVEYI AT 2.4 ANGSTROM RESOLUTION [J].
FISHER, AJ ;
RAUSHEL, FM ;
BALDWIN, TO ;
RAYMENT, I .
BIOCHEMISTRY, 1995, 34 (20) :6581-6586
[7]   PREPARATION AND INITIAL CHARACTERIZATION OF CRYSTALS OF THE PHOTOPROTEIN AEQUORIN FROM AEQUOREA-VICTORIA [J].
HANNICK, LI ;
PRASHER, DC ;
SCHULTZ, LW ;
DESCHAMPS, JR ;
WARD, KB .
PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1993, 15 (01) :103-107
[8]   CALCIUM-TRIGGERED LIGHT EMISSION IN RENILLA . A UNITARY BIOCHEMICAL SCHEME FOR COELENTERATE BIOLUMINESCENCE [J].
HASTINGS, JW ;
MORIN, JG .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1969, 37 (03) :493-+
[9]   CRYOCRYSTALLOGRAPHY OF BIOLOGICAL MACROMOLECULES - A GENERALLY APPLICABLE METHOD [J].
HOPE, H .
ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE, 1988, 44 :22-26
[10]   SEQUENCE OF THE CDNA-ENCODING THE CA2+-ACTIVATED PHOTOPROTEIN OBELIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA [J].
ILLARIONOV, BA ;
BONDAR, VS ;
ILLARIONOVA, VA ;
VYSOTSKI, ES .
GENE, 1995, 153 (02) :273-274
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