A review on native and denaturing purification methods for non-coding RNA (ncRNA)

被引：29

作者：

Kanwal, Fariha ^{[1
]}

Lu, Changrui ^{[1
]}

机构：

[1] DongHua Univ, Coll Chem Chem Engn & Biotechnol, 2999 North Ren Min Rd, Shanghai 201620, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2019年 / 1120卷

关键词：

Oligonucleotides purification; ncRNA; Urea-PAGE; Affinity purification; Liquid chromatography; HPLC; FPLC; PERFORMANCE LIQUID-CHROMATOGRAPHY; ION-EXCHANGE CHROMATOGRAPHY; SIGNAL RECOGNITION PARTICLE; LARGE-SCALE PREPARATION; SMALL INTERFERING RNA; IN-VITRO SELECTION; SYNTHETIC OLIGONUCLEOTIDES; TRANSCRIPTIONAL LANDSCAPE; AFFINITY PURIFICATION; GEL-ELECTROPHORESIS;

D O I：

10.1016/j.jchromb.2019.04.034

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, non-coding RNA (ncRNA) became the centerpiece of human genome research. Modern ncRNA-based research has revolutionized disease diagnosis and therapeutics. However, decoding structural/functional information of ncRNA requires large amount of pure RNA, and hence effective RNA preparation and purification protocols. This review focuses on purification schemes of synthetic oligonucleotides, particularly liquid chromatographic (LC) techniques as improved alternatives to urea-polyacrylamide gel electrophoresis (urea-PAGE) purification. Moreover, the review summarizes the shortcomings of urea-PAGE purification method and details the chromatographic purification such as affinity, ion-exchange (IE) or size-exclusion (SE) chromatography. Specifically, we discuss denaturing and native RNA purification schemes with newest developments. In short, the review evaluates nucleic acid purification schemes required for various structural analyses.

引用

页码：71 / 79

页数：9

共 144 条

[1] Evoking picomolar binding in RNA by a single phosphorodithioate linkage [J].

Abeydeera, N. Dinuka ;

Egli, Martin ;

Cox, Nehemiah ;

Mercier, Karen ;

Conde, Jonas Nascimento ;

Pallan, Pradeep S. ;

Mizurini, Daniella M. ;

Sierant, Malgorzata ;

Hibti, Fatima-Ezzahra ;

Hassell, Tom ;

Wang, Tianzhi ;

Liu, Feng-Wu ;

Liu, Hong-Min ;

Martinez, Carlos ;

Sood, Anil K. ;

Lybrand, Terry P. ;

Frydman, Chiraz ;

Monteiro, Robson Q. ;

Gomer, Richard H. ;

Nawrot, Barbara ;

Yang, Xianbin .

NUCLEIC ACIDS RESEARCH, 2016, 44 (17) :8052-8064

[2] Characterization of oligosorbents and application to the purification of ochratoxin A from wheat extracts [J].

Ali, Wassim Hadj ;

Pichon, Valerie .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (04) :1233-1240

[3]

Anderson AC, 1996, RNA, V2, P110

[4]

Andrus A, 2001, Curr Protoc Nucleic Acid Chem, VChapter 10, DOI 10.1002/0471142700.nc1005s01

[5]

Andrus A., 2000, Current Protocols in Nucleic Acid Chemistry, V1, p10.5.1, DOI DOI 10.15.11-10.15.13

[6]

Andrus A., 1998, HPLC Macromol, V872, P141, DOI [10.1093/oso/9780199635719.003.0006, DOI 10.1093/OSO/9780199635719.003.0006]

[7]

Avis JM, 2013, RECOMBINANT IN VITRO, P83

[8] The distributions, mechanisms, and structures of metabolite-binding riboswitches [J].

Barrick, Jeffrey E. ;

Breaker, Ronald R. .

GENOME BIOLOGY, 2007, 8 (11)

[9] Improved native affinity purification of RNA [J].

Batey, Robert T. ;

Kieft, Jeffrey S. .

RNA, 2007, 13 (08) :1384-1389

[10] Advances in methods for native expression and purification of RNA for structural studies [J].

Batey, Robert T. .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2014, 26 :1-8

← 1 2 3 4 5 6 7 8 9 10 →