A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons

被引：9

作者：

Kim, Beomsue ^{[2
]}

Feng, Suihan ^{[1
]}

Yun, Seong-Wook ^{[2
]}

Leong, Cheryl ^{[2
]}

Satapathy, Rudrakanta ^{[1
]}

Wan, Si Yan Diana ^{[2
]}

Chang, Young-Tae ^{[1
,2
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Med Chem Programme, Singapore 117543, Singapore

[2] Singapore Bioimaging Consortium, Lab Bioimaging Probe Dev, Singapore 138667, Singapore

来源：

CHEMBIOCHEM | 2016年 / 17卷 / 22期

关键词：

ABC transporters; Abcg2; cell recognition; CDg13; fluorescent probes; neural stem/progenitor cells; STEM-CELLS; CHEMICAL PROBE; BINDING;

D O I：

10.1002/cbic.201600490

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2(low) cells from heterogeneous cell populations.

引用

页码：2118 / 2122

页数：5

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